| Project/Area Number |
11671863
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
ONO Mitsunobu Hokkaido Univ., Graduate School of Dental Medicine, Inst., 大学院・歯学研究科, 助手 (50281829)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Motoaki Hokkaido Univ., Graduate School of Dental Medicine, Inst., 大学院・歯学研究科, 助手 (90239765)
HIGASHINO Fumihiro Hokkaido Univ., Graduate School of Dental Medicine, Inst., 大学院・歯学研究科, 助手 (50301891)
SHINDOH Masanobu Hokkaido Univ., Graduate School of Dental Medicine, Associate Prof., 大学院・歯学研究科, 助教授 (20162802)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | ets transcription factor E1AF / Oral squamous cell carcinoma / MMP / VEGF / Lymph node metastasis |
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| Research Abstract |
E1AF is an ets-oncogene family transcription factor. Previous reports have noted that E1AF can up-regulate transcription from multiple matrix metalloprotease (MMP) genes that confers invasive phenotype on human cancer cells. We have reported that the close correlation of E1AF expression and cancer cell malignancy by in vitro studies. This Implies that E1AF expression may contribute to predict the prognosis of patients with oral squamous cell carcinoma. In the present study, we have investigated the expression level of E1AF, MT1-MMP as well as vascular endothelial growth factor (VEGF) and evaluated the correlation to the metastatic potential in oral cancer patients.
Thirty-two cases of pathologically diagnosed as squamous cell carcinoma were investigated. All cases were T1 or T2 in the clinical staging. Lymph node metastasis was identified in 18 of the 32 cases examined. E1AF was positive 11 of the 32 cases, and lymph node metastasis was identified in 7 of the 11 E1AF positive cases (63.3 %). Five of the 32 cases were MT1-MMP positive, and all 5 cases were among the E1AF positive case. Four of the 5 MT1-MMP positive cases showed lymph node metastasis. VEGF was positive in 11 cases and lymph node metastasis was observed in 10 of the 11 VEGF positive cases (90.9%) that was statistically significant. There were 17 cases of which E1AF or VEGF was positive, and lymph node metastasis was identified in 12 of the 17 cases (70.6%). There were 15 cases in which E1AF and VEGF both were negative, and lymph node metastasis was identified in only 6 of these 15 cases (40%). These results indicate that the expression of E1AF and VEGF are important factors to predict the invasive and metastatic potential of oral squamous cell carcinomas.
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