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THE MODULATION OF IMMUNE AND INFLAMMATORY RESPONSES BY BENZODIAZEPINES VIA PERIPHERAL-TYPE BENZODIAZEPINE RECEPTORS

Research Project

Project/Area Number 11671865
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 病態科学系歯学(含放射線系歯学)
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MIYAWAKI Takuya  OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, ASSOCIATE PROFESSOR, 歯学部・附属病院, 助教授 (00219825)

Co-Investigator(Kenkyū-buntansha) KOHJITANI Atsushi  OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, ASSISTANT PROFESSOR, 歯学部・附属病院, 助手 (60304325)
MAEDA Shigeru  OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, LECTURER, 歯学部・附属病院, 講師 (50253000)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsBENZODIAZEPINE / MIDAZOLAM / CYTOKINE / INTERLEUKIN-6 / PERIPHERAL BLOOD MONONUCLEAR CELL / GENE EXPRESSION
Research Abstract

The purpose of this study is to evaluate the modulation of immune and inflammatory responses by benzodiazepines via peripheral-type benzodiazepine receptors. We investigated the effects of midazolam, a benzodiazepine, RO5-4864, a specific agonist of peripheral-type benzodiazepine receptors, clonazepam, a specific agonist of central-type benzodiazepine receptors, and PK11195, a specific antagonist of peripheral-type benzodiazepine receptors on interleukin-6 mRNA expression in human peripheral blood mononuclear cells (PBMCs).
The results demonstrated that :
1. Midazolam at the concentration of 10^<-4>〜10μg/ml dose-dependently inhibited the interleukin-6 mRNA expression in human PBMCs in 2 hours incubation at 37℃.
2. RO5-4864 and clonazepam at the concentration of 10μg/ml had no effect on the interleukin-6 mRNA expression in human PBMCs in 2 hours incubation at 37℃.
3. PK11195 did not reverse the inhibitory effect of midazolam on the interleukin-6 mRNA expression in human PBMCs.
These findings suggest that midazolam would modulate immune and inflammatory pesponse via another peripheral-type benzodiazepine receptor which is different from Ro5-4864 and PK11195 binding site.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Takuya Miyawaki, et al.: "The dose-dependent effects of midazolam on interlenkin-6 messenger RNA expression in human peripheral blood mononuclear cells in vitro."Anesthesiology. 91. A462 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takuya Miyawaki, et al.: "The dose-dependent effects of midazolam on interleukin-6 messenger RNA expression in human peripheral blood mononuclear cells in vitro."Anesthesiology. 91. A462 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takuya Miyawaki: "The dose-dependent effects of midazolam on interleukin-6 messenger RNA expression in human peripheral blood mononuclear cells in vitro"Anesthesiology. 91. A462 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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