THE MODULATION OF IMMUNE AND INFLAMMATORY RESPONSES BY BENZODIAZEPINES VIA PERIPHERAL-TYPE BENZODIAZEPINE RECEPTORS
| Project/Area Number |
11671865
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
MIYAWAKI Takuya OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, ASSOCIATE PROFESSOR, 歯学部・附属病院, 助教授 (00219825)
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| Co-Investigator(Kenkyū-buntansha) |
KOHJITANI Atsushi OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, ASSISTANT PROFESSOR, 歯学部・附属病院, 助手 (60304325)
MAEDA Shigeru OKAYAMA UNIVERSITY, HOSPITAL OF DENTISTRY, DENTALANESTHESIOLOGY, LECTURER, 歯学部・附属病院, 講師 (50253000)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | BENZODIAZEPINE / MIDAZOLAM / CYTOKINE / INTERLEUKIN-6 / PERIPHERAL BLOOD MONONUCLEAR CELL / GENE EXPRESSION |
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| Research Abstract |
The purpose of this study is to evaluate the modulation of immune and inflammatory responses by benzodiazepines via peripheral-type benzodiazepine receptors. We investigated the effects of midazolam, a benzodiazepine, RO5-4864, a specific agonist of peripheral-type benzodiazepine receptors, clonazepam, a specific agonist of central-type benzodiazepine receptors, and PK11195, a specific antagonist of peripheral-type benzodiazepine receptors on interleukin-6 mRNA expression in human peripheral blood mononuclear cells (PBMCs).
The results demonstrated that :
1. Midazolam at the concentration of 10^<-4>〜10μg/ml dose-dependently inhibited the interleukin-6 mRNA expression in human PBMCs in 2 hours incubation at 37℃.
2. RO5-4864 and clonazepam at the concentration of 10μg/ml had no effect on the interleukin-6 mRNA expression in human PBMCs in 2 hours incubation at 37℃.
3. PK11195 did not reverse the inhibitory effect of midazolam on the interleukin-6 mRNA expression in human PBMCs.
These findings suggest that midazolam would modulate immune and inflammatory pesponse via another peripheral-type benzodiazepine receptor which is different from Ro5-4864 and PK11195 binding site.
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Report
(3 results)
Research Products
(3 results)
