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Examination of the histological and bacteriological features of the interface between bacterial deposits and root dentin using a device that included exposed root dentin reproduced pulpal pressure in vitro.

Research Project

Project/Area Number 11671896
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionThe University of Tokushima, School of Dentistry

Principal Investigator

OZAKI Kazumi  Univ.of Tokushima, Conserv.Dent., Assistant professor, 歯学部・附属病院, 講師 (90214121)

Co-Investigator(Kenkyū-buntansha) KAWASAKI Akiko  Univ.of Tokushima, Conserv.Dent., Instructor, 歯学部, 助手 (60294708)
MATSUO Takashi  Univ.of Tokushima, Conserv.Dent., Professor, 歯学部, 教授 (30173800)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsCaries-associated bacteria / Pulpal pressure / Intracellular polysaccharide / 菌体関連多糖
Research Abstract

We evaluated structural change of the dentin and alteration of the localization and carbohydrate metabolism of bacteria in dental plaque and into dentinal tubules on the root dentin specimens in vitro and in situ, that we could observe with the progress in the time. In vitro experiments demonstrated that pH of the medium used for mixed culture with Streptococcus mutans and Lactobacillus casei was lower than it of the independent culture on a device which reproduced pulpal pressure by supplying the saline pressurized through dentinal tubules exposed in the pulpal side of root dentin specimens.
On microscope analysis, the numbers of bacterially infected dentinal tubules in specimens used for the mixed culture was significantly more abounding than it of the independent culture. By periodate-thiocarbohydrazide-silver proteinate-physical development (PTSP) staining method, which could detect the intracellular polysaccharide in bacterial cells, it was clarified that much intracellular polysaccharide exists in most bacteria within which exist in microcolonies of plaque layer nearest the dentin surface and in which invaded into dentinal-tubules.
On the other hand, in a root-dentin specimen, compulsorily exposed the surface and to the oral environment for only two months, the penetration of the bacteria into the dentinal tubules was visible, while in a twelve-month root-surface specimens in situ, some microcavities coalesced an indicated more wide-ranging cavities, but subsequent destruction of the dentin was not observed. The existence of mantle dentine was indicated as the factor by which brought about the difference of these results. It became clear to dynamically change in the time-dependent destruction of the dentin, which progresses on localization and abundance ratio of PTSP-positive-bacteria by means of image processing technology by the computer.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 尾崎和美 ら: "象牙質齲蝕に侵入した細菌種の同定-電顕用樹脂包埋試料から抽出した16S rDNAを用いて-"日本歯科保存学雑誌. 43(秋季特別号). 156-156 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 松尾敬志 ら: "根面齲蝕への対応・シールドレストレーションの応用"日本歯科評論. 臨時増刊号. 71-78 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉山昌宏 ら: "齲蝕象牙質へのシールド・レストレーションの可能性-細菌を封じ込める治療とその現在-"the Quintessence. 18・1. 77-89 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 尾崎和美 ら: "歯根部象牙質齲蝕誘発モデルの細菌学ならびに形態学的検索"日本歯科保存学雑誌. 42(春季特別号). 48-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Ozaki et al.: "A three-dimensional examination of morphological changes in experimental cementum caries lesions."Journal of Dental Research. 78(Special Issue). 411-411 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 綿城香 ら: "エナメル質形成不全症の1例"小児歯科学雑誌. 37・3. 620-625 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozaki K, et al.: "Bacterial identification using 16S rDNA extracted from resin-embedded carious dentin"Jpn J Conserv Dent. 43 (special issue). 156-156 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuo T, et al.: "Practical application of sealed restoration to root surface caries"Nippon Dental Review. Special issue. 71-78 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshiyama M.et. al.: "Possibility of sealed restoration to carious dentine ; Treatment of embedding bacteria and the present statue"the Quintessence. 18. 77-89 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozaki K, et al.: "Microbiological and histopathological observation of a radicular-dentin-specimen exposed to oral environment"Jpn J Conserv Dent. 42 (special issue). 48-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ozaki K, et al.: "A three-dimensional examination of morphological changes in experimental cementum caries lesions"Journal of Dental Research. 78 (special issue). 411-411 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wataki K, et al.: "Hereditary amelogenesis imperfecta : A case report"Journal of Pediatric Dentistry. 37. 620-625 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 尾崎和美 ら: "象牙質齲蝕に侵入した細菌種の同定-電顕用樹脂包埋試料から抽出した16S rDNAを用いて-"日本歯科保存学雑誌. 43(秋季特別号). 156-156 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松尾敬志 ら: "根面齲蝕への対応・シールドレストレーションの応用"日本歯科評論. 臨時増刊号. 71-78 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 尾崎和美ら: "歯根部象牙質齲蝕誘発モデルの細菌学ならびに形態学的検索"日本歯科保存学雑誌. 第42巻春季特別号. 48-48 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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