| Project/Area Number |
11671898
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HASHIGUCHI Isamu Faculty of Dental Science, KYUSHU UNIVERSITY, Assistant, 大学院・歯学研究院, 助手 (10150476)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Hidefumi Faculty of Dental Science, KYUSHU UNIVERSITY, Assistant, 大学院・歯学研究院, 助手 (10284514)
AKAMINE Akifumi Faculty of Dental Science, KYUSHU UNIVERSITY, Professor, 大学院・歯学研究院, 教授 (00117053)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Periapical periodontitis / Pulpititis / Nitric oxide / Macrophages / Ia antigen / Nerve fibers |
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| Research Abstract |
We examined immunohistochemically the distribution of macrophages, iNOS-positive cells, Ia antigen-positive cells and PGP9.5-imunoreactive nerve fibers in eperimentally induced rat pulpitis and periapical lesions. The results obtained were as follows.
1) In the pulp, macrophages increased in number from 0.5 days to 1 week after pulp exposure and disappeared 2 weeks after treatment. In the periapical periodontial tissues, macrophages increased until 4 weeks after treatment and decreased thereafter. The changes of PGP9.5-imunoreactive nerve fibers were closely related with the alteration in number of macrophages.
2) In the pulp, the number of iNOS-positive cells and Ia antigen-positive cells increased in the treated group much more than that in the control group until 1 week after treatment. However, the distribution of each cells was different , iNOS-positive cells were found near the abscess but Ia antigen-positive cells were a little remote from the abscess. In the periapical tissues, Ia antigen-positive cells increased 1 week after treatment and disappeared almostly thereafter. On the contrary, iNOS-positive cells were observed near the apical foramen between 2 weeks and 8 weeks.
Taken these findings into consideration, it was suggested that iNOS-positive cells might play a more important role than Ia antigen-positive cells in the front-line defense and seemed to have little relationship with periapical tissue destruction.
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