| Project/Area Number |
11671928
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
OKAZAKI Masayuki Hiroshima Univ., Fac. of Dent., Professor, 歯学部, 教授 (30107073)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Yasuhiro Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (90281162)
NOMURA Yuji Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (80218370)
WAKASA Kumio Hiroshima Univ., Fac. of Dent., Assoc. Prof., 歯学部, 助教授 (00136095)
SHIMADZU Atsushi Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (10274094)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Adhesion molecule / Apatites / Collagen / Laminated composites / Osteoblast |
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| Research Abstract |
Recently, the application of the concept of adhesion molecules to biomaterials was considered. In the present study, we designed a peptide containing the RGD-sequence, and the peptide was immobilized on the PEG-PS graft copolymer or conjugated on a collagen molecule, which modified the CO_3apatite-collagen composite. Then, the adhesive properties of osteoblasts onto the resulting composite were examined. The result demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Next task was to synthesize functionally graded CC_3apatite containing Mg, producing a negative gradient of magnesium concentration from the surface toward the core. The second task was to investigate the degree of cell adhesion to a composite that was made by mixing the FGMgCO_3apatite and collagen to facilitate bonding and processing. SEM observation showed that the osteoblast-like cells adhered well to the surface of the FGMgCO_3Ap-collagen composite. Staining with haematoxylin-eosin and alizarin red confirmed the existence of a great many more cells and a thicker extracellular matrix layer on the FGMgCO_3Ap-collagen composite than on the CO_3Ap-collagen composite. These results demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Animal experiments showed good biocompatibility of the composites.
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