Chemical Modification of Adhesion Motif onto Apatite-Collagen Laminated Composites

• Project/Area Number: 11671928
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: HIROSHIMA UNIVERSITY
• Principal Investigator: OKAZAKI Masayuki Hiroshima Univ., Fac. of Dent., Professor, 歯学部, 教授 (30107073)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Yasuhiro Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (90281162) ; NOMURA Yuji Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (80218370) ; WAKASA Kumio Hiroshima Univ., Fac. of Dent., Assoc. Prof., 歯学部, 助教授 (00136095) ; SHIMADZU Atsushi Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (10274094)
• Project Period (FY): 1999 – 2001
• Project Status: Completed(Fiscal Year 2001)
• Budget Amount: ¥3,400,000 (Direct Cost : ¥3,400,000); Fiscal Year 2001 : ¥600,000 (Direct Cost : ¥600,000); Fiscal Year 2000 : ¥700,000 (Direct Cost : ¥700,000); Fiscal Year 1999 : ¥2,100,000 (Direct Cost : ¥2,100,000)
• Keywords: Adhesion molecule / Apatites / Collagen / Laminated composites / Osteoblast

Research Abstract

Recently, the application of the concept of adhesion molecules to biomaterials was considered. In the present study, we designed a peptide containing the RGD-sequence, and the peptide was immobilized on the PEG-PS graft copolymer or conjugated on a collagen molecule, which modified the CO_3apatite-collagen composite. Then, the adhesive properties of osteoblasts onto the resulting composite were examined. The result demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Next task was to synthesize functionally graded CC_3apatite containing Mg, producing a negative gradient of magnesium concentration from the surface toward the core. The second task was to investigate the degree of cell adhesion to a composite that was made by mixing the FGMgCO_3apatite and collagen to facilitate bonding and processing. SEM observation showed that the osteoblast-like cells adhered well to the surface of the FGMgCO_3Ap-collagen composite. Staining with haematoxylin-eosin and alizarin red confirmed the existence of a great many more cells and a thicker extracellular matrix layer on the FGMgCO_3Ap-collagen composite than on the CO_3Ap-collagen composite. These results demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Animal experiments showed good biocompatibility of the composites.

Research Products

• [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37. 95-100 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M.et al.: "Synthesis of functionally graded MgCO_3apatite accelerating osteoblast adhesion"J Biomed Mater Res. (in press). (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M. Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials."Dentistry in Japan. 37. 95-100 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M. et al.: "Synthesis of functionally graded MgCO_3apatite accelerating osteoblast adhesion."J Biomed Mater Res. (in press). (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37. 95-100 (2001)
• [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M.et al.: "Synthesis of functionally graded MgCO_3apatite acceleratimg osteoblast adhesion"J Biomed Mater Res. (in press). (2002)
• [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37(in press). (2001)