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Chemical Modification of Adhesion Motif onto Apatite-Collagen Laminated Composites

Research Project

Project/Area Number 11671928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OKAZAKI Masayuki  Hiroshima Univ., Fac. of Dent., Professor, 歯学部, 教授 (30107073)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Yasuhiro  Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (90281162)
NOMURA Yuji  Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (80218370)
WAKASA Kumio  Hiroshima Univ., Fac. of Dent., Assoc. Prof., 歯学部, 助教授 (00136095)
SHIMADZU Atsushi  Hiroshima Univ., Fac. of Dent., Assistant, 歯学部, 助手 (10274094)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsAdhesion molecule / Apatites / Collagen / Laminated composites / Osteoblast
Research Abstract

Recently, the application of the concept of adhesion molecules to biomaterials was considered. In the present study, we designed a peptide containing the RGD-sequence, and the peptide was immobilized on the PEG-PS graft copolymer or conjugated on a collagen molecule, which modified the CO_3apatite-collagen composite. Then, the adhesive properties of osteoblasts onto the resulting composite were examined. The result demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Next task was to synthesize functionally graded CC_3apatite containing Mg, producing a negative gradient of magnesium concentration from the surface toward the core. The second task was to investigate the degree of cell adhesion to a composite that was made by mixing the FGMgCO_3apatite and collagen to facilitate bonding and processing. SEM observation showed that the osteoblast-like cells adhered well to the surface of the FGMgCO_3Ap-collagen composite. Staining with haematoxylin-eosin and alizarin red confirmed the existence of a great many more cells and a thicker extracellular matrix layer on the FGMgCO_3Ap-collagen composite than on the CO_3Ap-collagen composite. These results demonstrated the acceleration effect of magnesium ions on osteoblast adhesion on the FGMgCO_3Ap-collagen composite. Animal experiments showed good biocompatibility of the composites.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37. 95-100 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M.et al.: "Synthesis of functionally graded MgCO_3apatite accelerating osteoblast adhesion"J Biomed Mater Res. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials."Dentistry in Japan. 37. 95-100 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M. et al.: "Synthesis of functionally graded MgCO_3apatite accelerating osteoblast adhesion."J Biomed Mater Res. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37. 95-100 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamasaki, Y., Yoshida, Y., Okazaki, M.et al.: "Synthesis of functionally graded MgCO_3apatite acceleratimg osteoblast adhesion"J Biomed Mater Res. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Okazaki et al.: "A new concept of CO_3apatite-collagen composites with adhesion motif as biomaterials"Dentistry in Japan. 37(in press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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