|Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Although glycosaminoglycans (GAG) clearly play an important role in wound healing, there have been few reports on changes in GAG during osseous healing around porous implants. We investigated histochemically and immunohistochemically the location of GAG around implants using 15-week-old Sprague-Dawley rats. Porous implants 1.0 mm in diameter and 3.0 mm long were inserted into the thighbones. The animals were sacrficed 1, 2, 3, 5 and 10 weeks after the operation. Deparaffinized sections were histochemically stained with toluidine blue after hyaluronidase digestion. Other deparaffinized sections were treated with 2B6 (for chondroitin 4 sulfate), 6B6 (for dermatan sulfate) antibodies, and stained by the indirect immunoperoxdase technique.
Hyaluronic acid was identified on tissues around the implant at 1 week. Chondoitin sulfate and calcification were identified after 2 weeks in the peri-implant tissue. After 3 weeks, dermatan sulfate was identified in the peri-implant tissue, and calcification had become active. There was strong reaction for GAG in the first and third weeks. These results indicate that large amounts of GAG were produced within the first three weeks after the implant operation, and that there was a close connection between calcification and osseous healing around the implant.