| Project/Area Number |
11671972
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
NISHIMURA Taiichi ASAHIKAWA MEDICAL COLLEGE, DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY, ASSISTANT PROFESSOR, 医学部, 講師 (30113749)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEKAWA Masanori ASAHIKAWA MEDICAL COLLEGE, DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY, ASSISTANT, 医学部, 助手 (50216876)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | osteoblast / proliferation / differentiation / alkaline phosphatase activity / mineralized bone nodules |
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| Research Abstract |
1. Effects of radiation on cell growth
(1) In the control culture, the osteoblasts isolated from the calvaria of newborn rats proliferated rapidly after initial plating and reached confluence (day 0). Then they continued to proliferate and the number of osteoblasts peaked on day 21.
(2) Cell proliferation was inhibited by irradiation on day 4, 11, and 18 in a dose-dependent fashion.
2. Effects of radiation on ALP activity
(1) In control culture, ALP activity increased in a time-dependent manner.
(2) ALP activity decreased in a dose-derendent fashion after irradiation on day 4 and did not recover to the control level.
(3) ALP activity in cells irradiated on day 11 was significantly higher than that in the control cells at day 21 and 28. A similar tendency was observed in cells irradiated on day 18.
3. Effects of radiation on formation of mineralized bone nodules
(1) In the control culture, the area of mineralized bone nodules increased in a time-dependent manner.
(2) The area of mineralized bone nodules was significantly decreased by irradiation on day 4.
(3) The area of mineralized bone nodules was significantly decreased by 15 Gy of irradiation on day 11.
(4) Only 5 Gy of irradiation on day 18 significantly increased the area of mineralized bone nodules at day 28.
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