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Application of octacalcium phosphate for bone substitutes and a carrier for cytokines.

Research Project

Project/Area Number 11671974
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTohoku University

Principal Investigator

MOTEGI Katsutoshi  Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (60013984)

Co-Investigator(Kenkyū-buntansha) SASANO Yasuyuki  Tohoku University, Graduate School of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (30196191)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsBone / Bone Formation / Bone Repair / Octacalcium Phosphate / Calcium Phosphate / サイトカイン
Research Abstract

Our previous study reported that synthetic octacalcium phosphate (OCP) enhances bone repair if implanted in rat skull defects. We hypothesized that OCP can be used as an effective carrier for transforming growth factor-β_1 (TGF-β_1) and fibroblast growth factor (FGF) promote bone repair. The present study was designed to investigate histomorphometrically whether combination with recombinant human TGF-β_1 or FGF could promote bone repair caused by OCP per se (Control/OCP). A full-thickness standardized trephine defect was made in the rat parietal bone and OCP combined with recombinant human TGF-β_1 (TGF-β_1/OCP), OCP combined with recombinant human FGF (FGF/OCP) or Control/OCP was implanted into the defect. The experimented rats from each group were fixed at 2, 4, and 8 weeks after implantation. Histomorphometrical analysis of the percentage of newly formed bone (n-Bone%) and remaining implants (r-Imp%) in the defect was performed.
The statistical analysis showed the n-Bone% of TGF-β_1/OCP was significantly higher than that of the Control/OCP in week 4, while the r-Imp% of TGF-β_1/OCP was significantly lower than that of the Control/OCP.The present study demonstrated that OCP can be used as an effective carrier for TGF-β_1 and their combination enhance bone repair as well as resorption of the carrier OCP in the early stage of bone formation.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Sasano Y, et al.: "Implanted octacalcium phosphate(OCP)stimulates osteogenesis by osteoblastic cells and/or committed osteoprogenitors in rat calvarial periosteum."Anat Rec. 256. 1-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamakura S, et al.: "Implantation of octacalcium phosphate(OCP)in rat skull defects enhances bone repair."J Dent Res. 78. 1682-1687 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasano Y, et al.: "Immunohistochemical localization of type I collagen, fibronectin and tenascin C during embryonic osteogenesis in the dentary of mandibles and tibias in rats."Histochem J. 32. 591-598 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasano Y, et al.: "Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles."Anat Embryol. 202. 31-37 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katou F, et al.: "Immunological activation of dermal Langerhans cells in a model of human inflammed skin."Am J Pathol. 156. 519-527 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamakura S, et al.: "Implantation of octacalcium phosphate nucleates isolated bone formation in rat skull defects."Oral Dis. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasano Y, Kamakura S, Homma H, Suzuki O, Mizoguchi I, Kagayama M: "Implanted octacalcium phosphate (OCP) stimulates osteogenesis by osteoblastic cells and/or committed osteoprogenitors in rat calvarial periosteum."Anal Rec. 256. 1-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamakura S, Sasano Y, Homma H, Suzuki O, Kagayama M, Motegi K: "Implantation of octacalcium phosphate (OCP) in rat skull defects enhances bone repair."J Dent Res. 78. 1682-1687 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasano Y, Li H-C, Zhu J-X, Imanaka-Yoshida K, Mizoguchi I, Kagayama M: "Immunohistochemical localization of type I collagen, fibronectin and tenascin C during embryonic osteogenesis in the dentary of mandibles and tibias in rats."Histochem J. 32. 591-598 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasano Y, Zhu J-X, Kamakura S, Kusunoki S, Mizoguchi I, Kagayama M: "Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles."Anat Embryol. 202. 31-37 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katou F, Ohtani H, Saaristo A, Nagura H, Motegi K: "Immunological activation of dermal Langerhans cells in a model of human inflammed skin"Am J Pathol. 156. 519-527 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamakura S, Sasano Y, Homma H, Suzuki O, Kagayama M, Motegi K.: "Implantation of octacalcium phosphate nucleates isolated bone formation in rat skull defects."Oral Dis. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamakura S, et al.: "Implantation of octacalcium phosphate nucleates isolated bone formation in rat skull defects."Oral Dis. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasano Y, et al.: "Gene and protein expressions of type I collagen are regulated tissue-specifically in rat hyaline cartilages in vivo."Eur J Morphol. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katou F, et al.: "Immunological activation of dermal Langerhans cells in a model of human inflammed skin."Am J Pathol. 156. 519-527 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasano Y, et al.: "Immunohistochemical localization of type I collagen, fibronectin and tenascin C during embryonic osteogenesis in the dentary of mandibles and tibias in rats."Histochem J. 32. 591-598 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sasano Y, et al.: "Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles."Anat Embryol. 202. 31-37 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kagayama M, et al.: "Confocal microscopy of Tomes' granular layer in dog premolar teeth."Anat Embryol. 201. 131-137 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kamakura S.et al.: "Implantation of octacalcium phosphate (OCP) in rat skull defects enhances bone repair"Journal of Dental Research. 78. 1682-1687 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sasano Y.et al: "Implanted octacalcium phosphate (OCP) stimulates osteogenesis by osteoblastic cells and/or committed osteoprogenitors in rat calvarial perioseum"Anatomical Record. 256. 1-6 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kagayama M.et al.: "Confocal microscopy of dentinal tubules in human tooth stained with alizarin red"Anatomy and Embryology. 199. 233-238 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sasano Y.et al.: "Expression of major bone extracellular matrix proteins during embryonic osteogenesis in rat mandibles"Anatomy and Embryology. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato S.et al.: "Long-term changes in clinical signs and symptoms and disc position and morphology in patients with nonreducing disc displacement in the temporomendibular joint"J Oral Maxillofac Surg. 57. 23-29 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sato S.et al.: "Disc position and morphology in patients with nonreducing disc displacement treated by injection of sodium hyaluronate"Int J Oral Maxillofac Surg. 28. 253-257 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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