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Periosteal regeneration and bone augmentation using recombinant human BMP-2/atelocollagen onlay implant

Research Project

Project/Area Number 11672000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

MURATA Masaru  Health Sciences University of Hokkaido, Second Department of Oral and Maxillofacial Surgery, Lecturer, 歯学部, 講師 (00260662)

Co-Investigator(Kenkyū-buntansha) ARISUE Makoto  Health Sciences University of Hokkaido, Second Department of Oral and Maxillofacial Surgery, Professor, 歯学部, 教授 (20091407)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBMP / Collagen / Periosteum / Bone augmentation / Regeneration / Implant / 骨再生
Research Abstract

The efficacy of limited exposure of bone marrow by cortical perforations were evaluated in subperiosteal rhBMP-2 implant system. Ten μg of rhBMP-2 solution and 10 mg of pepsindigested collagen (atelocollagen) solution were mixed in the sterilized tube and lyophilized. The composition of rhBMP-2 (10 μg)/collagen (10 mg) was implanted into the space between the periosteum and skull with or without outer cortical perforations in 10-month old Wistar rats. At 1, 2, 3 and 4 weeks after operation, the implant and the skull were removed en bloc and decalcified coronal sections (4 μm in thickness) were made and stained with hematoxylin and eosin and Elastica van Gieson. RhBMP-2/collagen implant with cortical perforations resulted in an initial acceleration of osteogenesis, whereas the rhBMP-2/collagen without perforations resulted in peripheral bone with many round empty spaces in the middle area at 1 and 2 weeks. In addition, the rhBMP-2/collagen implant was completely replaced by newly formed bone at 4 weeks. Our present results indicate that limited cortical petforations to host bed contributed to promote osteogenesis in initial stages and this novel rhBMP-2/atelocollagen implant system, an absorbable device replaced by new bone, would lead to improved and more predictable treatments for bone augmentation.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Masaru Murata: "Bone augmentation by recombinant human BMP-2 and collagen on adultrat parietalbone."Int.J.Oral Maxillofac.Surg.. 28. 232-237 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masaru Murata: "Bone augmentation by onlay implant using recombinant human BMP-2 and collagen on adultrat skull without periosteum."Clin.Oral Impl.Res.. 11. 289-295 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 村田勝: "組織工学と遺伝子治療の融合による骨再生"歯界展望. 95. 960-963 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masaru Murata, Bing-Zhen Huang, Toshiyuki Shibata, Sawako Imai, Noriyuki Nagai, Makoto Arisue: "Bone augmentation by recombinant human BMP-2 and collagen on adult rat parietal bone"Int.J.Oral Maxillofac.Surg. 28. 232-237 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masaru Murata, Fumiyo Maki, Daisuke Sato, Toshiyuki Shibata, Makoto Arisue: "Bone augmentation by onlay implant using recombinant human BMP-2 and collagen on adult rat skull without periosteum."Clin.Oral Impl.Res.. 11. 289-295 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masaru Murata: "Bone regeneration by combination of tissue engineering and gene therapy."DENTAL OUTLOOK. 95. 960-963 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Masaru Murata: "Bone augmentation by recombinant human BMP-2 and collagen on adultrat parietalbone."Int.J.Oral Maxillofac.Surg.. 28. 232-237 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masaru Murata: "Bone augmentation by onlay implant using recombinant human BMP-2 and collagenon adultrat skull without periosteum."Clin.Oral Impl.Res.. 11. 289-295 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 村田勝: "組織工学と遺伝子治療の融合による骨再生"歯界展望. 95. 960-963 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masaru Murata: "Bone augmentation by recombinant human BMP-2 and collagen on adult rat parietal bone"Int.J.Oral Maxillofac.surg.. 28. 232-237 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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