α2 integrin expression in cyclosporin A-induced gingival overgrowth in rats
| Project/Area Number |
11672083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | University of Tokushima |
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Principal Investigator |
KATAOKA Masatoshi Univ.of Tokushima School of Dentistry Univ.Dental Hospital, Assistant Proffesor, 歯学部・附属病院, 講師 (20224438)
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| Co-Investigator(Kenkyū-buntansha) |
ASAHARA Yoji Univ.of Tokushima School of Dentistry, Research Associate, 歯学部, 助手 (30294713)
KIDO Jun-ichi Univ.of Tokushima School of Dentistry, Associate Proffesor, 歯学部, 助教授 (10195315)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Cyclosporin A / Gingival overgrowth / Collagen phagocytosis / α2 integrin |
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| Research Abstract |
Cyclosporin-A (CsA) is used as an immunosuppressive agent and its side effect is the induction of fibrous gingival overgrowth. We have shown that experimental gingival overgrowth in rat is induced by a decrease of type I collagen degradation in the connective tissue, predominantly through a reduced collagen phagocytosis by fibroblasts (J.Cell.Physiol., vol.183, 2000). The α2β1 integrin have been described as type I collagen receptor on fibroblasts and the α2 subunit plays a critical role in the phagocytic regulation of collagen internalization. The relationship between collagen phagocytic activity and expression of α2 integrin on fibroblasts in CsA-induced gingival overgrowth was investigated. Fibroblasts were isolated from mandibular molar gingiva of rats fed with a powdered diet containing or lacking CsA for 8 or 30 days. In vitro analysis was performed to measure the phagocytosis of cultured fibroblasts with flow cytometry using FITC-labeled collagen coated latex beads. Fibroblasts isolated from CsA-treated rat on days 8 and 30 contained 2.1±1.0% and 13.0±3.5% phagocytic cells, whereas control fibroblasts contained 13.7±2.7% and 33.3±4.9% phagocytic cells, respectively. Immunostaining with FITC-labeled anti-rat α2 integrin antibody revealed that fibroblasts isolated from CsA-treated rat were weakly stained than those of each control. The quantitative analysis of α2 integrin expression using flow cytometry revealed that fluorescence intensity of fibroblasts isolated from CsA-treated rat on days 8 and 30 were 25.1±6.1 and 51.1±2.5, whereas 50.7±6.3 and 67.0±5.5 in the controls, respectively. RT-PCR analysis revealed that α2 integrin mRNA expressions were suppressed by CsA than those of each control. These findings suggest that the decrease of collagen phagocytosis due to the lower expression of α2 integrin on fibroblasts are closely associated with the increase of type I collagen in CsA-treated rat gingiva.
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Report
(3 results)
Research Products
(4 results)
