| Project/Area Number |
11672085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
GOTO Yasuharu Faculty of Dental Science, KYUSHU UNIVERSITY, Assistant, 大学院・歯学研究院, 助手 (00170473)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZA Takayoshi Faculty of Dental Science, KYUSHU UNIVERSITY, Assistant, 大学院・歯学研究院, 助手 (80304814)
HIRATA Masako Dental Hospital, Assistant, 歯学部・附属病院, 助手 (10153769)
AKAMINE Akifumi Faculty of Dental Science, KYUSHU UNIVERSITY, Professor, 大学院・歯学研究院, 教授 (00117053)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | junctional epithelial cells / inducible nitric oxide synthase / NF-kappa B / substance P / neutrophils / rats / immunohistochemistry / periodontal disease / LPS |
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| Research Abstract |
The junctional epithelium (JE) is affected earlier than oral sulcular epithelium (OSE) and oral epithelium (OE) by stimuli that evoke periodontal inflammation. Nitric oxide (NO) has a variety of biological activities and is thought to modulate inflammation. NO is generated from L-arginine by NO synthases (NOS), classified into neural-type NOS (nNOS), endothelial-type (eNOS) and inducible-type (iNOS). The transcription factor NF-kappa B plays a critical role in the transcriptional regulation of iNOS gene. In this study we investigated the immunohistochemical expression of iNOS and eNOS and NF-kappa B in rat gingiva. And we also investigated the effect of neuropeptide substance P (SP) on expression of iNOS in JE cells.
The immunoreactive products of iNOS, eNOS and NF-kappa B were found throughout various cells (JE cells, OSE cells, OE cells, fibroblasts and endothelial cells) in the gingiva. In the gingiva, 30 min after SP application at concentrations of 10-4 M, the effect of SP on expression of iNOS in JE cells was not detected immunohistochemically. It was suggested that JE cells might produce NO and form a line of defense against harmful stimuli such as bacteria. Further studies are needed to determine the role of NO in JE cells.
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