| Project/Area Number |
11672088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Showa University |
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Principal Investigator |
MIYAZAWA Yasushi Showa University, Dental School, lecturer (90219775)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYSHI Makoto Showa University, Dental School, lecturer (80186767)
OKAMATSU Yoshimasa Showa University, Dental School, Assistant professor (50286845)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Human gingival fibroblasts / Thy-1 antigen / cell function / heterogeneity / Thy-1 / IFN-γ / IL-4 |
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| Research Abstract |
Local immune and inflammatory responses may be regulated through human gingival fibroblasts(HGF), which are correlated with the tissue rgeneration on periodontal desease. An emerging concept is that fibroblast heterogeneity has recently been appreciated with the cell morphology, function and expression of various antigens on the cell surface. Thy-1 antigen is a highly glycosylated cell surface glycoprotein with a molecular weight of about 35kDa and is anchored to the cell membrane via linkage to phosphatidylinositol in mouse. On the other hands, in human cells. Thy-1 expression is also indicated to neuronal cells and fibroblasts. There are different results concerning the morphology and cell function of Thy-1 expression on lung fibroblasts. In present study, we examined that HGF could be separated into Thy-1 positive cell [Thy-1(+)]and Thy-1 negative cell[Thy-1(-)] subpopulations that defined morphologic or functional differences including collagen production in vivro. Parental HGF were 10-40% positive by immunofluorescence analysis. Then, separation of parental fibroblasts into Thy-1(+) and Thy-1(-) subsets were accomplished using three to four rounds of magnetic beading and FACS system. Distinct morphologic differences exist between HGF that display Thy-1 and those that do not. At the microscopic level, the Thy-1(+) cells possessed more elongated and extended projections than did the Thy-1(-)Thy-1(-) cells, which were more spread and rounded. Once HGF were separated into subsets, their Thy 1 phenotype remained stable over culture throughout experiments passage 4 to 10. Furthermore, Thy-1(+) HGF line has tendency producing more total collagen and non-collagen protein, compared to the Thy-1(-) line. In conclusions, predominant proliferation of Thy-1(+) HGF in periodontal tissue may has more advantage in the healing or regeneration rates of periodontal tissue destructions in vivo.
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