• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Theoretical prediction of fluorescent characteristics and development of fluorogenic derivatization reagents

Research Project

Project/Area Number 11672137
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

SANTA Tomofumi  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Associate professor, 大学院・薬学系研究科, 助教授 (30187306)

Co-Investigator(Kenkyū-buntansha) IMAI Kazuhiro  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (50012620)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsbenzofurazan / fluorescence / semi-empirical molecular orbital calculation / HPLC / derivatization reagent / 誘導体化
Research Abstract

We have already established the relationship between the fluorescence characteristics of 4,7-disubstituted benzofurazan compounds and Hammett substituent constants of the substituent groups. In this research, we studied the relationship between the fluorescence characteristics and energies of molecular orbitals of benzofurazan compounds by using the semi-empirical molecular orbital calculation method.
At first, the compounds having the large difference of the energy between the lowest singlet excited state and the higher triplet excited state showed the strong fluorescent intensity, giving the prediction method of the fluorescent characteristics of the benzofurazan compounds by the theoretical molecular orbital calculation.
Next, we developed the fluorogenic derivatization reagents, PSBD-NCO for alcohols, AABD-SH for carboxylic acids, ES-ABD-F, water-soluble reagents, for peptides, and MTBD-NCS for peptides sequencing analysis.
As a result, we made clear the relationship between the fluorescence characteristics and the structure of benzofurazan compounds and we developed the fluorogenic reagents based on the relationship.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Tomofumi Santa: "A new fluorogenic reagent for carboxylic acids, 7-acetylamino-4-mercapto-2,1.3-benzoxadiazaole (AABD-SH),"Analyst. 124. 1689-1693 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "A fluorogenic reagent, 7-phenylsulfonyl-4-(2,1,3-benzoxadiazolyl) isocyanate for alcohols, with development based on the empirical method for prediction."Analytical Chemistry. 71. 5367-5371 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "Semi-empirical PM3 calculation predict the fluorescence quantum yields of 4-monosubstituted benzofurazan compounds."J.Chem.Soc.Perkin Trans 2. 1199-1207 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "Study on the fluorescent on-off properties of benzofurazan compounds bearing aromatic substituent group and design of fluorescent on-off derivatization reagents."Analyst. 125. 1839-1845 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshi Kajiro: "Development of water-soluble fluorogenic reagents having a 2,1,3-benzoxadiazole structure and their application to the determination of peptides."Analyst. 125. 1115-1121 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akira Toriba: "Develoment of an amino acid sequence and D/L-configuration determination method of peptide with a new fluorescence Edman reagent."Analytical Chemistry. 72. 732-739 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomofumi Santa: "A new fluorogenic reagent for carboxylic acids, 7-acetylamino-4-mercapto-2,1.3-benzoxadiazaole (AABD-SH), derived from an empirical method forpredicting fluorescence characteristics."Analyst. 124. 1689-1693 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "A fluorogenic reagent, 7-phenylsulfonyl-4-(2,1,3-benzoxadiazolyl) isocyanate for alcohols, with development based on the empirical method for prediction."Analytical Chemistry. 71. 5367-5371 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "Semi-empirical PM3 calculation predict the fluorescence quantum yields of 4-monosubstituted benzofurazan compounds."J.Chem.Soc.Perkin Trans. 2. 1199-1207 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiichi Uchiyama: "Study on the fluorescent on-off properties of benzofurazan compounds bearing aromatic substituent group and design of fluorescent on-off derivatization reagents."Analyst. 125. 1839-1845 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshi Kajiro: "Development of water-soluble fluorogenic reagents having a 2,1,3-benzoxadiazole structure and their application to the determination of peptides."Analyst. 125. 1115-1121 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akira Toriba: "Development of an amino acid sequence and D/L-configuration determination method of peptide with a new fluorescence Edman reagent."Analytical Chemistry. 72. 732-739 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akira Toriba: "Development of an amino acid sequence and D/L-configuration determination method of peptide with a new fluorescence Edman reagent."Analytical Chemistry. 72. 732-739 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Seiichi Uchiyama: "Semi-empirical PM3 calculation predict the fluorescence quantum yields of 4-monosubstituted benzofurazan compounds."J.Chem.Soc.Perkin Trans 2. 1199-1207 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Toshi Kajiro: "Development of water-soluble fluorogenic reagents having a 2,1,3-benzoxadiazole structure and their application to the determination of peptides."Analyst. 125. 1115-1121 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Seiichi Uchiyama: "Study on the fluorescent on-off properties of benzofurazan compounds bearing aromatic substituent group and design of fluorescent on-off derivatization reagents."Analyst. 125. 1839-1845 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Seiichi Uchiyama: "Semi-empirical PM3 calculation reveals the relationship between the fluorescence characteristics of 4,7-disubstituted benzofurazan compounds"J. Chem. Soc. Perkin Trans 2. 569-576 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Seiichi Uchiyama: "Fluorescence characteristies of six 4,7-disubstituted benzofurazan copounds : an empirical and semi-empirical MO study"J. Chem. Soc. Perkin Trans 2. 2525-2532 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Seiichi Uchiyama: "A fluorogenic reagent, 7-phenylsulfonyl-4-(2,1,3-benzoxadiazolyl)isocyanate for alcohols, with development based on the empirical method for prediction"Analytical Chemistry. 71. 5367-5371 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomofumi Santa: "A new fluorogenic reagent for carboxylic acids, 7-acetylamino-4-mercapto-2,1.3-benzoxadiazaole (AABD-SH)"Analyst. 124. 1689-1693 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi