| Project/Area Number |
11672253
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Teikyo University School of Medicine |
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Principal Investigator |
TAKANO Takako Teikyo Univeesity School of Medicine, Associate Professor, 医学部, 助教授 (50236246)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANOUCHI Yasuko Teikyo Univeesity School of Medicine, Assistant, 医学部, 助手 (40246038)
TAKANO Kaoru j. University of Tsukuba, Institute of Materials Science, Associate Professor, 物質工学系, 助教授 (60133005)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | high-pressure / cell death / apoptosis / lymphoblats / HL-60 / caspase-3 / tubulin / heat shock protein / 微小管 / caspase |
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| Research Abstract |
The human lymphoblasts (EB3) and human promyelocytic leukemia cells (HL-60) pressurized for 30 minutes at 37℃ were investigated. The pressure at which 50% cell viability of HL-60 is attained was lower than that of EB3. While, in EB3 cells, apoptotic cells began to be observed from 8 hours after decompression at 100 MPa, in HL-60 cells, they were observed immediately after decompression. In EB3 cells, a high-pressure signal overexpressed p53, which induced caspase-3 processing. In HL-60 cells, phospholylation of p38 MAPK was induced by high-pressure, participating in the common caspase cascade. The pressure-induced attenuation in α-tubulin and β-tubulin expression was observed after pressure treatment of 100 MPa for 30 minutes both by Western blot analysis and by 2-dimentional gel electrophoresis. We proposed that microtubules play a role in signal transduction executing apoptosis. The expression of several proteins increased immediately after decompression by 2-dimentional gel electrophoresis. One of them was revealed HSP60 (heat shock protein 60). HSP60 may mediate folding reaction under the high-pressure stress.
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