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General Research associated with Tumor cell desruction, especially dendritic cells

Research Project

Project/Area Number 11672300
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionUniversity of the Ryukyus

Principal Investigator

ARAKI Koichi  University of the Ryukyus, Faculty of Medicine, Dept. of Hematoimmunology, Professor, 医学部, 教授 (40040604)

Co-Investigator(Kenkyū-buntansha) NAKAMOTO Kazumi  University of the Ryukyus, Faculty of Medicine, Dept. of Hematoimmunology, Assistant, 医学部, 助手 (30244338)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsDendritic cell / CD34 / CD1a / CD11c / CD83 / CDw123 / GM-CSF / SCF / dendritic cell / IL-3 / Stem cell / MACS cell separation / Dendritic Cell / Stem Cell
Research Abstract

Dendritic Cells are the most potent antigen presenting cells that initiate primary immune responses. The aims of this study are to analyze the composition of umbilical cord blood cells and to examine the characteristics of dendritic cells before and after culture. Umbilical cord blood cells were freshly determined the cell surface antigen, and then after culturing for 1, 2, 3, and 4 weeks with GM-CSF, IL-3 and SCF, every week they were determined the antigens. Method of flowcytometry was used to determine the number of dendritic cells and cell surface antigens before and after culture by using monoclonal antibody. Monoclonal antibodies included CD4, CD8, CD19, CD34, Cd83, CD1a, CD11c and CDw123. In the fresh umbilical cord blood, CD4^+ cells are 48.1%, CD8^+ 19.6, CD19^+ 7.5%, CD34^+ 2.9%, CD38^+ 73.5%, CD83^+ 1.5%, CD1a^+ 0.3% and CD11c^+ 12.2%. By the way, in fresh umbilical cord blood cells, total cells of CD83^+ were 1.9±1.1x10^5/ml, CD1a^+ 0.3±0.2x10^5/ml, CD11c^+ 5.9±2.5x10^5/ml and CDw123^+ 2.7±2.2x10^5/ml. On the other hand, after having cultured for 3 weeks, total cells of CD CD83^+ were 9.2±5.2x10^5/ml, CD1a^+ 9.8±9.3x10^5/ml, CD11c^+ 23.2±11.5x10^5/ml and CDw123^+ 19.6±10.1x10^5/ml.
As characteristic of umbilical cord blood after having cultured with cytokines of SCF, GM-CSF and IL-3, average population of lymphocytes decreased gradually, while average numbers of monocytes increased gradually in population. By culture with cytokine, dendritic cells increased up 3.1%-53.4%, and 6.9-28.2x10^5/ml in actual numbers. In comparison with fresh cord blood and their three-week cultured blood, CD83^+ active numbers were about 5.4 times, CD1a^+ 36.7 times, CD11c^+ 4.8 times and CDw123^+ 6.8 times. Umbilical cord blood cells cultured with cytokines differentiated into CD1a^+, CD83^+, CD11c^+ and CD83^+ dendritic cells. It seems these dendritic cells are very effective as antigen presenting cell and for cancer immunotherapy.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 仲本和美, 小谷由香, 荒木弘一: "ヒト臍滞血の性状について"臨床血液. 42. 1047 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 仲村久美子, 荒木弘一, 仲本和美: "フローサイトメトリーによる臍滞血に含まれる免疫担当細胞の検討"平成13年度琉球大学医学部保健学科卒業研究論文集. No.29. 225-228 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 大西雄介, 荒木弘一, 仲本和美: "Balb c/X63 担癌マウスにおける免疫担当細胞の動態-脾臓と血液"平成14年度琉球大学医学部保健学科卒業研究論文集. No.30. 177-180 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 與座剛, 荒木弘一, 仲本和美: "Balb c/X63 担癌マウスにおける免疫担当細胞の動態-胸腺と骨髄"平成14年琉球大学医学部保健学科卒業研究論文集. No.30. 173-176 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lian S, Nakamoto K, Nakamura K, Araki K.: "Chracteristics of dendritic cells with CD1a+,CD11c+,CD83+,and CDw123+ in umbilical cord blood"China-Japan Medical Conference 2002 program book. (口頭発表). 159 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamot K, Kotani Y, Araki K.: "Characteristics of human umbilical cord blood"The Japanese Journal of Clinical Hematology. 42-10. 1047 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura K, Araki K, Nakamot K.: "Immunocompetent cells in Umbilical Cord Blood by Flow Cytometry"Thesis for bachelor of Health Sciences, School of Health Sciences, Univ. of the Ryukyus. No 29. 225-228 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Onishi Y, Araki K, Nakamot K.: "Movement of Immunocompetent cells in Spleen and Blood of Balb C/X63 Mice"Thesis for bachelor of Health Sciences, School of Health Sciences, Univ. of the Ryukyus. No 30. 177-180 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoza T, Araki K, Nakamot K.: "Changes of Immunocompetent cells in Thymus and Bone Marrow of Balb C/X63 Mice"Thesis for bachelor of Health Sciences, School of Health Sciences, Univ. of the Ryukyus. No 30. 173-176 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lian S, Nakamoto K, Nakamura K, Araki K.: "Characteristics of dendritic cells with CD1a^+, CD11c^+, CD83^+, and CDw123^+ in umbilical cord blood (Poster Presentation), in China-Japan Medical Conference 2002 program book"159 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tsukasaki, K.Araki, et al.: "Deoxycoformycin-Containing Combination Chemotherapy for Adult T-cell Leukemia-lymphoma"Int.J.Hemat.. 77・2. 164-170 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Yamada, K.Araki, et al.: "A new G-CSF-supported combination Chemotherapy, LSG15, for adult T-cell leukemia-lymphoma : Japan Clinical Oncology Group Study 9303"Brit.J.Haemat.. 113. 375-382 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Araki,K.Nakamokto: "Clinical significans of serum soluble IL-2R levels in patients with adult T-cell leukemia (ATL) and HTLV-I carriers."Clin.Exp.Immunol.. 119. 259-263 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 荒木弘一,仲本和美,他: "HTLV-I endemic area沖縄における成人T細胞白血病(ATL)の発症状況"Ryukyu Med.J.. 18(4). 149-154 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 荒木弘一,仲本和美,他: "HTLV-Iキャリアの沖縄と札幌における血液塗抹標本の検討"癌の臨床. 45(4). 349-355 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Kobayashi,K.Araki,et al.: "Long-term Follow-up Results of Adult Patients with Acute Lymphocytic Leukemia or Lymphoblastic Lymphoma Treated with Short-term,Alternating Non-cross-resistant Chemotherapy:Japan Clinical Oncology Group Study 8702."Jpn.J.Clin.Oncol.. 29(7). 340-348 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Takenaka,K.Araki,et al.: "Altemating Combination Chemotherpy COP(Cyclophosphamide,Vincristine,Prednisone)and MP(Melphalan,Prednisone)in Multiple Myeloma:Multicenter PhaseII Study(JCOG8906)."Jpn.J.Clin.Oncol.. 29(10). 485-489 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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