| Project/Area Number |
11672311
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
KAKU Mitsuo Tohoku University, Graduate School of Medicine, Professor (40224357)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Keiko Tohoku University, Tohoku University Hospital, Lecturer (00291253)
KANEMITSU Keiji Tohoku University, Tohoku University Hospital, assistant professor (90277971)
SIMADA Jingoro Saint Marianna University, Department of Microbiology, Professor (50056701)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Streptococcus pneumoniae / penicillin resistant / pneumolysin / neuraminidase / production of virulence factor / adherent activity / capsule |
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| Research Abstract |
We performed the research on epidemiology and virulence of penicillin resistant Streptococcus pneumoniae (PRSP). The incidence rate of PRSP is highly 47% among S.pneumoniae isolates. Capsular serotypes of PRSP are different from those of penicillin sensitive S.pneumoniae (PSSP) and main capsular serotype of PRSP is type 19. The capsule producibility, the adherent activity to human respiratory cell and the producibility of neuraminidase & pneumolysin of PRSP shows the equality with those of PSSP and the clinical significance of PRSP is strongly recognized. We evaluated the inhibitory effects of antimicrobial agents on the production of neuraminidase and pneumolysin by PRSP. Beta-lactam group antibiotics show no inhibitory effect of the production of neuraminidase and pneumolysin. Macrolide group antibiotics, Lincomycin group antibiotics, Tetracycline group antibiotics, Aminoglycoside group antibiotics and Fluoroquinolone group antibiotics show the inhibitory effect of the production of pneumolysin. Especially, Macrolide, Lincomycin and Tetracycline group antibiotics show the strong inhibitory effect of the production of pneumolysin at sub-MICs. These results indicate that Macrolide, Lincomycin and Tetracycline group antibiotics have strong virulence suppressing effects and these antimicrobial agents are expected for the useful agents for severe infectious disease by PRSP.
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