| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Research Abstract |
Using 3 kinds of pigments, the reaction which used the dye by the mixed system was examined. The decoloration rate constant increases more, as the oxidation-reduction potential is smaller. It is considered that the reason why the rate constant differs is because the dye space structure differs, though oxidation-reduction potential is equal. In the dye mixed system, it was not effective for the reaction between Orange I and HRP, even if Orange II and Orange G coexist, and the reaction of Orange I rapidly progressed. In the meantime, Orange II slowed down, and Orange G quickened. It seems to be a cause that the radical dye generation rate differs on this by the difference between enzymatic substrate specificity and dye oxidation-reduction potential. Therefore, the reaction mechanism of anticipated dye mixed system consists like the following. In case of the dye mixed system, since Orange I of the reaction is very rapider than that of other dye, the radical is formed from the right after reaction, and it decomposes afterwards. In the meantime, the reaction of Orange II does not start, since the Orange II oxidation-reduction potential is high, while Orange I is reacting with the enzyme prior. The reaction of Orange II starts after the reaction end of Orange I.It is also similar that reaction start of Orange G is late. It is based that the reaction rate of Orange II is also slower than the single system by mixed system on following reasons. In doing the role of the activator of Orange G, the orange II radical receives the electron in Orange G.The orange II radical returns in Orange II.It is considered that in the dye mixed system, the reaction of Orange II is suppressed further than the Orange II single system.
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