| Project/Area Number |
11680147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | NAKAMURA GAKUEN UNIVERSITY, FACULTY OF HOME ECONOMICS |
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Principal Investigator |
MATSUGUMA Miki Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 助手 (40259669)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Ryoko Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 助手 (90271436)
FUJITA Mamoru Nakamura Gakuen University, Faculty of Home Economics Professor, 家政学部, 教授 (60037471)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | food allergen / ultrastructure / intestine / suckling rat / 吸収上皮細胞 |
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| Research Abstract |
Con A-HRP, PNA-HRP, LCA-HRP and RCA_<120>-HRP bound strongly to components of the glycocalyx on the plasma membrane of the microvilli, apical coated pits and the small tubular pits at the base of the microvilli of the absorptive cells. The lectin-conjugates on the plasma membranes were endocytosed from the coated pits and small tubular pits, and then transported into coated vesicles, vesicles and small tubules. Lectin-HRP conjugates were later found within the intercellular space. In contrast, SBA-HRP and DBA-HRP bound weakly to the plasma membranes. The absorptive cells demonstrated little uptake or transepithelial transport.
When WGA-HRP was injected into the intestinal lumen in vivo, the jejunal absorptive cells formed a deep and wide apical invagination at the base of the microvilli. WGA-HRP bound strongly to the glycocalyx on the plasma membranes however was almost entirely transported into the early endosomes, late endosomes, multivesicular bodies and lysosomes.
These studies suggest the similarity and diversity of invasion of allergen in the small intestinal absorptive cells of the suckling rats.
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