Elimination of Oxidized Guanine Ribonucleotides in Mammalian Cells
| Project/Area Number |
11680551
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HAYAKAWA Hiroshi Department of Medical Biochemistry Graduate School of Medical Sciences Kyushu University, Research Associate, 大学院・医学研究院, 助手 (70150422)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Oxidative stress / RNA binding protein / Transcriptional error / Erroneous protein / 突然変異 / ヌクレオチド代謝 / RNA結合因子 |
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| Research Abstract |
The persistence of the oxidized guanine residue, 8-oxo-7, 8-dihydroguanine (8-oxoguanine), in mRNA would cause translational errors. Eschericha coli cell has a protein that binds specifically to RNA containing 8-oxoguanine. The protein was identified by two dimensional gel analysis and the mutants coding the protein are hyperresistant to paraquat, a drug that induces oxidative stress in the cell. Erroneous protein synthesis due to oxidation of guanine in the substrate nucleotide and RNA, may be prevented by actions of MutT^1 and the proteins, the former degrading 8-oxoguanine-containing ribonucleoside triphosphate while the latter eliminates 8-oxoguanine-containing RNA from translation process.
'SCIENCE 278, 128 (1997)
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Report
(3 results)
Research Products
(7 results)
