| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
The persistence of the oxidized guanine residue, 8-oxo-7, 8-dihydroguanine (8-oxoguanine), in mRNA would cause translational errors. Eschericha coli cell has a protein that binds specifically to RNA containing 8-oxoguanine. The protein was identified by two dimensional gel analysis and the mutants coding the protein are hyperresistant to paraquat, a drug that induces oxidative stress in the cell. Erroneous protein synthesis due to oxidation of guanine in the substrate nucleotide and RNA, may be prevented by actions of MutT^1 and the proteins, the former degrading 8-oxoguanine-containing ribonucleoside triphosphate while the latter eliminates 8-oxoguanine-containing RNA from translation process.
'SCIENCE 278, 128 (1997)
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