Organization of porphyrin derivatives using photosynthetic light-havesting polypeptide and its model polypeptide
| Project/Area Number |
11680587
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
NANGO Mamoru Nagoya Institute of Technology, faculty of engineering, professor, 工学部, 教授 (90109893)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | photosynthesis / antenna complex / chlorophyll derivatives / self-molecular assembly / lipid bilayers / アンテナ系タンパク質 / ポルフィリン誘導体 / モデルポリペプチド / 人工アンテナ系 |
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| Research Abstract |
The light-harvesting (LH) -a and -b polypeptides of photosynthetic bacteria organize a bachteriochlorophyll a (BChl a) complex according to cooperative interactions between the LH polypeptides and BChl a in a biologial membrane so that an efficient energy transfer between bacteriochlorophylls may occur in the LH complex. Work from several laboratories has demonstrated self-assemblies of porphyrins by using synthetic polypeptides to organize artificial hemoprotein models. However, there has been little study of molecular assembly of chlorophylls by using synthetic polypeptides to organize an artificial LH complex. Here, molecular assemblies of bacteriochloropyll a (BChl a) and its model compounds using light-harvesting (LH) polypeptide separately isolated from photosynthetic bacteria, Rhodospirllum rubrum (Rsp. rubrum) and its model synthetic polypeptides were examined in lipid bilayers as well as in n-Octyl-b-D-glucopyranoside (OG) micelle. Comparing the amino acid sequence in the N-and C-terminal segments of the LH polypeptide and its model peptides on the complex-formation with Zn-BChl a or BChl a reveals that the amino acid residues from M to F in the N-terminal segment of the LH polypeptide essentially account for the difference in the complex-formation between Zn-BChl a and BChl a. Interestingly, self-assembly of BChl a and its model compounds using the LH polypeptide and its model polypeptides in lipid bilayers was observed, analogous to an artificial LH 1-type complex. It is interesting to note that BChl a and its analogues complexes can be organized according to cooperative interactions between the LH polypeptide or its model polypeptides and bachteriochlorophyll derivatives.
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Report
(3 results)
Research Products
(26 results)
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[Publications] A.Kashiwada, Y.Takeuchi, H.Watanabe, T.Mizuno, H.Yasue, K.Iida, Z.-Y.Wang, T.Nozawa, H.Kawai, T.Nagamura, Y.Kurono and M.Nango: "Molecular Assembly of Covalently-linked Mesoporphyrin Dimers with Light-harvesting Polypeptides"Tetrahedron Lett.. 2115-2119 (2000)
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