| Project/Area Number |
11680694
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
SUGIYAMA Shin Graduate School of Science, Nagoya University, Assistant Professor, 大学院・理学研究科, 助手 (00270984)
|
|---|
| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Yeast / Drosophila / Cell Cycle |
|---|
| Research Abstract |
The GCC(Growth ＆ Cell Cycle)-A1 gene was first identified in D. melanogaster as a mutant showing slow growth and reduced body size. Hypomorphic adults have small bristles, shortened lifespans and reduced male fertilty. Eggs produced by hypomorphic females show arrested nuclear divisions at cleavage stage. Twin-spot analysis revealed a reduction in clone size according to allelic strength. These results suggest a role in cell proliferation or cell growth. Over-expression of GCC-A1 in the eye produced a rough-eye phenotype in a dose dependent manner. Genetic interaction with cell death genes indicate this superficial phenotype is caused by apoptosis. Over-expression of GCC-A1 combined with expression of P35 to suppress apoptosis led to an increase in the number of ommatidia. Over-expression of GCC-A1 in the wing disc caused an increase in cell size, suggesting that GCC-A1's primary role may be in cell growth. GCC-A1's putative protein product is novel with no motifs revealing its biochemical function, but a database search found genes of unknown function in C. elegans, S. cerevisiae and S. pombe sharing homology restricted to a domain of approximately 150 amino acids. We disrupted the 4 genes in S. cerevisiae which we refer to as GCC-A, -B1, -B2 and -C. GCC-A disruptants are arrested at G1 phase and GCC-C disruptants show slow growth and do not sporulate suggesting that GCC type proteins function in cell cycle progression in both insects and yeast. Analysis of tagged proteins showed that both fly GCC and yeast GCC-A are localized at membrane structures in the cytoplasm suggesting further similarities in the roles of the genes in both organisms.
|
