| Project/Area Number |
11680753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kanazawa University |
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Principal Investigator |
YOKOYAMA Shigeru Kanazawa University Graduate School of Medicine, Department of Biophysical Genetics, Associate Professor, 大学院・医学系研究科, 助教授 (00210633)
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHIDA Haruhiro Kanazawa University Graduate School of Medicine, Department of Biophysical Genetics, Professor, 大学院・医学系研究科, 教授 (30093066)
HOSHI Naoto Kanazawa University Graduate School of Medicine, Department of Biophysical Genetics, Research Associate, 大学院・医学系研究科, 助手 (90229170)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | voltage-gated potassium channel / Kv1.1 / Kv1.2 / 膜電位依存性K+チャネル / 膜電位依存性^+チャネル / 膜電位依存性K^+チャネル |
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| Research Abstract |
Kv1.1 and Kv1.2, two most closely related members of the Shaker-like gene subfamily, encode pore-forming subuaits of voltage-gated potassium (K^+) channels. To clarify physiological roles of these proteins in primary sensory afferentiation, we have performed inimunohistochemical analysis using specific antidodies. Immunoperoxidase staining of dorsal root and trigeminal ganglia revealed that strong immunoreactivity (IR) for Kv1.1 and Kv1.2 was predominant in medium- and large-sized neurons. In double-immunofluorescence experiments, the cells strongly positive for these subunits were most frequently observed in RT97 (neurofilament)-positive large neurons; but rarely in peripherin- or IB4-positive small neurons. In the spinal dorsal horn, both the aati-Kv1.1 and anti-Kv1.2 antibodies heavily staiaed the deeper laminae III-IV. At the electron microscopic level, IRs for these proteins were preferentially localized in myelinated axons with large diameter. These results suggest that voltage-gated K^+ channels composed of Kv 1.1 and/or Kv 1.2 subunits may play important roles in conducting mechanoceptive and proprioceptiye sensation from skin and muscles. Conceivably, decreased expression of these channels after injury might cause hypereexcitability of neurons, thereby leading to hyperalgegia or allodyaia.
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