• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of mammalian circadian rhythms and Per1 transcriptional oscillation using Per1 tramsgenicm

Research Project

Project/Area Number 11680780
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe University of Tokyo

Principal Investigator

TEI Hajime  Institute of Medical Science, University of Tokyo. Research Assails, 医科学研究所, 助手 (00242115)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsPeriod1 / Clock genes / Circadian rhythms / 行動リズム
Research Abstract

Many biochemical, physiological and behavioral processes in many organisms exhibit circadian rhythms. Circadian rhythms are driven by autonomous oscillators and entrained by daily light-dark cycles. The transcription of Per1, a mammalian clock gene, oscillates in a circadian manner in the mouse suprachiasmatic nucleus (SCN ; the central pacemaker of the mammalian circadian clock) with a peak in the daytime and a trough at night. In addition, the expression of mPer1 in the SCN is induced immediately by a light pulse even at night. Function of the circadian expression of the mammalian Per1 gene is a key question for the regulation of circadian rhythms. For elucidation of the molecular mechanisms controlling the mammalian circadian clock, the genomic sequences of the human and mouse Per1 genes in addition to their transcriptional start sites have been determined. Both of the genomic sequences consist of 23 exons spanning approximately 16 kb. Comparisons of both genes revealed five and one … More conserved segments in the 5' flanking regions and the first introns, respectively. These conserved segments contained several potential regulatory elements such as five E-boxes (the binding site for the Clock-Bmal1 complex). Transfection analyses using a series of deletion and point mutants of the mPer1 : : luc reporter showed that each of the five E-boxes was functional for the Per1 induction mediated by Clock and Bmal1. Second, we constructed transgenic rat lines with constitutive expression of the mouse Per1 gene using elongation 1 alpha or neural specific enolase promoters. Both the circadian period of locomoter activity and entrainment to light- dark cycles were severely affected in several transgenic lines. In addition, we measured the expression of the native (rat) Per1 and Per2 genes in the SCN and retina of the transgenic lines under DD conditions. The circadian expression of endogenous Per1 and Per2 genes was diminished in the transgenic lines. These results clearly indicate that the circadian expression and light induction of the mammalian Per1 gene is involved in rhythm generation and/or entrainment of the circadian clock. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Akiyama,M.,Moriya,T.,Takahashi,S.,Kouzou,Y.,Wakamatsu,H.,Maetani,M.,Watanbe,S.,Tei,H.,Sakaki,Y.and Shibata S.: "Inhibition of Light- or Glutamate-Induced mPer1 Expression Represses the Phase Shifts into the Mouse Circadian Locomoter Suprachiasmatic Firing Rhythms."J.Neuroscience. 19. 1115-1121 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hida,A.,Koike,N.,Hirose,M.,Hattori,M.,Sakaki,Y.and Tei,H.: "The human and mouse Period1 genes : five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription."Genomics. 65. 224-233 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamazaki,S.,Numano,R.,Abe,M.,Hida,A.,Takahashi,R.,Ueda,M.,Block,G.D.,Sakaki,Y.,Menaker,M.and Tei,H.: "Resetting Central and Peripheral Circadian Oscillators in Transgenic Rats."Science. 288. 682-685 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Stokkan K-A.,Yamazaki S.,Tei,H.,Sakaki Y.,and Menaker M.: "Entrainment of the Circadian Clock in the Liver by Feeding."Science. 291. 490-493 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiyama, M., Moriya, T., Takahashi, S., Kouzou, Y., Wakamatsu, H., Maetani, M., Watanbe, S., Tei.H., Sakaki, Y.and Shibata S.: "Inhibition of Light-or Glutamate-Induced mPer1 Expression Represses the Phase Shifts into the Mouse Circadian Locomoter Suprachiasmatic Firing Rhythms."J.Neuroscience. 19. 1115-1121 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hida, A., Koike, N., Hirose, M., Hattori, M., Sakaki, Y.and Tei, H.: "The human and mouse Period1 genes : five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription."Genomics. 65. 224-233 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamazaki, S., Numano, R., Abe, M., Hida, A., Takahashi, R., Ueda, M., Block, G.D., Sakaki, Y., Menaker, M.and Tei, H.: "Resetting Central and Peripheral Circadian Oscillators in Transgenic Rats."Science. 288. 682-685 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Stokkan K-A., Yamazaki S., Tei H., Sakaki Y., and Menaker M.: "Entrainment of the Circadian Clock in the Liver by Feeding."Science. 291. 490-493 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hida,A.,Koike,N.,Hirose,M.,Hattori,M.,Sakaki,Y.and Tei,H.: "The human and mouse Period1 genes : five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription."Genomics. 65. 224-233 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamazaki,S.,Numano,R.,Abe,M.,Hida,A.,Takahashi,R.,Ueda,M.,Block,G.D.,Sakaki,Y.,Menaker,M.and Tei,H.: "Resetting Central and Peripheral Circadian Oscillators in Transgenic Rats."Science. 288. 682-685 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Stokkan K-A.,Yamazaki S.,Tei H.,Sakaki Y.,and Menaker M.: "Entrainment of the Circadian Clock in the Liver by Feeding."Science. 291. 490-493 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akiyama,M.: "Inhibition of Light-or Glutamate-Induced mPerl Expression Represses the Phase Shifts into the Mouse Circadian Locomoter Suprachiasmatic Firing Rhythms"J.Neuroscience. 19. 1115-1121 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hida,A.: "The human and mouse Period1 genes : five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription"Genomics. (in Press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamazaki,S.: "Central and Peripheral Circadian Oscillators in Transgenic Rats"Science. (in Press). (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi