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Analysis of neuronal circuit for the gonadotropin-releasing hormone surege generator (GnRH) using an in vitro GnRH surge generator model

Research Project

Project/Area Number 11680790
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionYokohama City University

Principal Investigator

FUNABASHI Toshiya  Yokohama City University School of Medicine Associate Professor, 医学部, 講師 (70229102)

Co-Investigator(Kenkyū-buntansha) MITSUSHIMA Dai  Yokohama City University School of Medicine Assistant Professor, 医学部, 講師 (70264603)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
KeywordsRat / GnRH surge generator / Vasopressin / co-culture / Preoptic area / GABA neurons / acute slice / Suprachiasmatic nucleus / エストロジェン / LHサージ / バソプレッシン / 培養 / 視索前野 / 視交叉上核 / カルシウムイメージング / イメージング / サージジェネレーター / GnRH
Research Abstract

1. We determined morphological changes in an in vitro gonadotropin-releasing hormone (GnRH) surge generator model we developed. GnRH neurons in the POA slice were clearly visible 4 weeks after co-culture with the suprachiasmatic nucleus (SCN). However, we could not stained vasopressin neurons. We tried to identify living GnRH neurons by transfecting a expression vector containing GnRH promoter and Ds-Red as a reporter. Transfection efficiency of this vector is, however, very low even in GT1-7 cell lines.
2. We investigated the propagation of electrical stimulus-evoked excitation in the in vitro GnRH surge generator model. We found that the evoked excitation was not propagated to the POA slice when an electrical stimulus was applied in the SCN slice, suggesting that the POA slice could not make synaptic contact with the SCN slice.
3. We next measured GnRH releases in tne POA slice obtained from the adult rat on the day of proestrus. Bicuculline, an GABA_A receptor antagonist, significantly increased GnRH releases in the POA slice. Interestingly, when the POA slice was perfused with the SCN slice, we found that GnRH release was significantly fractuated, suggesting that GnRH release was conveyed by the SCN via a humoral information.
From these results, we conclude that an acute slice preparation is useful tools to investigate the GnRH surge generator activity.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Funabashi T, Aiba S, Sano A, Shinohara K, Kimura F: "Intracerebroventricular injection of arginine-vasopressin V1 receptor antagonist attenuates the surge of luteinizing hormone and prolactin secretion in proestrous rats"Neuroscience Letters. 260. 37-40 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Shinohara K, Mitsushima D, Kimura F: "Gonadotropin-releasing hormone exhibits circadian rhythm in phase with arginine-vasopressin in co-cultures of the female rat preoptic area and suprachiasmatic nucleus"Journal of Neuroendocrinology. 12. 521-528 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 舩橋利也, 貴邑冨久子: "内側視索前野GnRHニューロンと性周期"Clinical Neuroscience. 19. 1122-1124 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Mitsushima D, Nakamura IT, Shinohara K, Suyama K, Kimura F: "Gonadotropin-releasing hormone (GnRH) surge generator in female rats"Progress in Brain Research (special issue for 2nd international symposium on the comparative biology of GnRH). (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Shinohara K, Kimura F: "Neuronal control circuit for the gonadotropin-releasing hormone surge in rats. In / Neuroplasticity, development, and steroid hormone action"Handa RJ, Hayashi S, Terasawa E, Kawata M(des) CRC Press, Florida. 405 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Aiba S, Sano A, Shinohara K, Kimura F: "Intracerebroventricular injection of arginine-vasopressin V1 receptor antagonist attenuates the surge of luteinizing hormone and prolactin secretion in proestrous rats"Neuroscience Letters. 260. 37-40 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Shinohara K, Mitsushima D, Kimura F: "Gonadotropin-releasing hormone exhibits circadian rhythm in phase with arginine-vasopressin in co-cultures of the female rat preoptic area and suprachiasmatic nucleus"Journal of Neuroendocrinology. 12. 521-528 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Shinohara K, Kimura F: "Neuronal control circuit for the gonadotropin-releasing hormone surge in rats. In Neuroplasticity, development, and steroid hormone action (Handa RJ, Hayashi S, Terasawa E, Kawata M, eds)"CRC Press Florida. 169-176 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Mitsushima D, Nakamura JT, Shinohara K, Suyama K, Kimura F: "Gonadotropin-releasing hormone (GnRH) surge generator in female rats"Progress in Brain Research. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Funabashi T, Shinohara K, Mitsushima D and Kimura F: "Gonadotropin-releasing horm one exhibits circadian rhythm in phase with arginine-vasopressin in co-cultures of the female rat preoptic area and suprachiasmatic nucleus"J Neuroendocrinol. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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