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Analysis of nociceptin-induced spontaneous transient outward currents and their physiological function in the CNS

Research Project

Project/Area Number 11680811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionKumamoto University (2000-2001)
Kansai Medical University (1999)

Principal Investigator

SHIRASAKI Tetsuya  Kumamoto Univ., Pharmaceutical Sci., Associate Prof., 薬学部, 助教授 (30264047)

Co-Investigator(Kenkyū-buntansha) MATSUDA Hiroko  Kansai Med. Univ., Medicine, Professor, 医学部, 教授 (10181736)
SUGIMOTO Tetsuo  Kansai Med. Univ., Medicine, Professor, 医学部, 教授 (90144352)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsNociceptin / STOCs / Intracellular Ca^<2+> / Hippocampus / Dentate Gyrus / ORL1 / Ca^<2+> Sparks / Ca^<2+> Quarks / Ca^<2+> / SMOCs / SMDCs / ORLI
Research Abstract

Spontaneous transient outward currents (STOCs) have been found in peripheral neurons and smooth muscle cells, but rarely in central neurons. Using a nystatin-perforated patch clamp technique, we succeeded in recording STOCs in mouse dentate gyrus granule cells. Nociceptin increased the amplitude and frequency of STOCs. We consider modulation of STOCs to be a new means to regulate cell activity in central neurons, and studied their characteristics and mechanism of augmentation. The whole-cell current-voltage relationship showed outward rectification and the reversal potential was close to the equilibrium potential for K The frequency of STOCs increased at depolarized potentials. Tetraethylammonium, iberiotoxin and a Ca^<2+> chelator BAPTA-AM inhibited STOCs. These results suggest the involvement of large-conductance Ca^<2+>-activated K^+ channels. Single-channel recordings in the inside-out configuration revealed Ca^<2+>-activated K^+ channels with a conductance ranging from 82 to 352 p … More S. The augmenting effect of nociceptin was cancelled by [Phe^1Ψ(CH -NH)Gly^2]Nociceptin(1-13)NH. Cd^<2+> did not affect the transient outward currents or augmentation by nociceptin. Whereas nociceptin, theophylline and cyclic ADP ribose induced transient outward currents with short duration observed under control conditions, inositol 1, 4, 5-trisphosphate induced transient outward currents with long duration, in addition to those with short duration. Ryanodine inhibited nociceptin from augmenting STOCs. Our data suggest that Ca^<2+> sparks, not Ca^<2+> quarks, transiently activate large-conductance Ca -activated K^+ channels to induce transient outward currents Nociceptin probably sensitizes ryanodine receptors and increases transient outward currents to reduce cell excitability. Nociceptin-induced increase in the amplitude and frequency of STOCs was observed even in the nociceptin receptor knockout mice, suggesting the presence of receptor subtype. In this study, we first revealed the presence and the characteristics of STOCs in the CNS and the modulation of STOCs by G protein coupled receptor. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Shirasaki T, Houtani T, Sugimoto T, Matsuda H: "Spontaneous transient outward currents : modulation by nociceptin in murine dentate gyrus granule cells"Brain Research. 917. 191-205 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T Shirasaki, T Houtani, T Sugimoto and H Matsuda: "Spontaneous transient outward currents : modulation by nociceptin in murine dentate gyrus granule cells"Brain Research. 917. 191-205 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shirasaki T, Houtani T, Sugimoto T, Matsuda H: "Spontaneous transient outward currents : modulation by nociceptin in murine dentate gyrus granule cells"Brain Research. 917. 191-205 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tetsuya Shirasaki et al.: "Spontaneous transient outward currents and modulation by nociceptin in mice hippocampal dentate granule cells."Neuroscience Research. 24. S80 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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