| Project/Area Number |
11680813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
神経・脳内生理学
|
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| Research Institution | OKAZAKI NATIONAL RESEARCH INSTITUTES |
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Principal Investigator |
NAKAI Junichi NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES, DEPARTMENT OF INFORMATION PHYSIOLOGY, OKAZAKI NATIONAL RESEARCH INSTITUTES, RESEARCH ASSOCIATE, 生理学研究所, 助手 (80237198)
|
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | EXCITATION-CONTRUCTION COUPLING / RYANODINE RECEPTOR / CALCIUM CHANNEL / カルシウム放出チャネル |
|---|
| Research Abstract |
Voltage-gated L-type calcium channels (Dihydropyridine receptors) and ryanodine receptors (calcium release channels) play important roles in muscle contruction. In this research, chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) ryanodine receptor were expressed in HEK293 cells to assess their calcium dependence in [^3H] ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in calcium activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions corresponding RyR2 regions can result in loss and/or reduction of [^3H] ryanodine binding affinity while maintaining channel activity.
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