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Functional relationship between transgene and the endogenous host gene in mice

Research Project

Project/Area Number 11680821
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MOHRI Shirou  Graduate School of Medical Sciences, KYUSHU UNIVERSITY Prof, 大学院・医学研究院, 教授 (40117271)

Co-Investigator(Kenkyū-buntansha) ISHIZU Akihiro  Graduate School of Medical Sciences, KYUSHU UNIVERSITY Research Assistant, 大学院・医学研究院, 助手 (90243930)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordstransgenic mice / gene function / prion protein / transgene / endogenous host gene / 導入遺伝子発現
Research Abstract

To clarify a functional relationship between transgene and the endogenous host gene in transgenic mice (Tg), we investigate susceptibility of prion model Tg to human prion. The three strains of mice which express various level of mouse/human chimeric prion protein (ChPrP) and different endogenous murine prion protein (MoPrP), that is, Prnp-w/w, w/0, 0/0. Twenty micro-liters of 10% brain homogenate of sporadic CJD patient (codon129met/met) was inoculated to the mice with intracerebral route. The susceptibility was estimated by measuring incubation periods of affected mice. Prnp-0/0 mice was highest susceptibility to human prion and Prnp-w/0 was middle, but Prn-w/w was less susceptibility in every strain of mice.
The result indicate that endogenous host MoPrP seemed to repress the functional expression of transgene in mice. We always have to evaluate function of the transgene in Tg considering with endogenous host genes.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Doh-ura K.,Mohri S. et al.: "Brain injury does not modify transmissible spongiform encephalopathy caused by intraperitoneal inoculation with Fukuoka-1 strain."J Gen.Virol.. 80. 1551-1556 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Matsuda H., Mitsuda H., Nakamura N., Furusawa S., Mohri S.and Kitamoto T.: "A chickin monoclonal antibody with specificity for the N-terminal of human prion protein."FEMS Immuno.Med.Microbiol.. Vol. 13. 189-194 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Doh-ura K.,Mohri S. et al.: "Brain injury does not modify transmissible spongiform encephalopathy caused by intraperitoneal inoculation with Fukuoka-1 strain."J Gen.Virol.. 80. 1551-1556 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Doh-ura K., Mohri S.et al.: "Brain injury does not modify transmissible spongiform encephalopathy caused by intraperitoneal inoculation with Fukuoka-1 strain"J Gen.Virol.. 80. 1551-1556 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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