| Project/Area Number |
11680822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagasaki University |
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Principal Investigator |
SATO Hiroshi Nagasaki University, School of Medicine, Professor, 医学部, 教授 (50072947)
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| Co-Investigator(Kenkyū-buntansha) |
KATAMINE Shigeru Nagasaki University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (40161062)
OHSAWA Kazutaka Nagasaki University, School of Medicine, Assistant Professor, 医学部, 助手 (90244756)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Parainfluyenza virus / Guinea pigs / Paramyxovirus / PIV3 / Experimental animals / Rats / ウイルス / マウス |
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| Research Abstract |
In order to obtain the pathological disease model in rats and guinea pigs, we attempted the infection experiments using PIV3. The animal experiments were carried out which made immune deficient state. Afterwards dynamic image of the immunological status and viral genome detection were followed after infection.
C243 strain of HPIV3 and GP8 strain of GPFV3 were inoculated i.n. to animals. The experiment animals (4 week-old male rats : SD and guinea pigs : Hartley) were administrated cyclosporin A (CsA : i.m.; 15mg/KgBW/day) daily to established immunosuppressive condition. Following results were obtained.
1. Infection experiments revealed that the virus genome could be detected in pulmonary region of guinea pigs at least day 8 through 10 post-infection, on the other hand during day 2 and 8 in immunosuppressed rats.
2. Humoral immunity conducted by IFA method was not detected during examination period in guinea pigs. Rats, however, showed IFA antibody on day 10 post-infection in spite of the treatment with cyclosporin A for immunosuppression.
3. Although the consolidation was demonstrated in guinea pig lungs, precise examination should be continuously followed.
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