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disease model of parainfluenza virus type 3 derived from guinea pigs on rats and guinea pigs

Research Project

Project/Area Number 11680822
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNagasaki University

Principal Investigator

SATO Hiroshi  Nagasaki University, School of Medicine, Professor, 医学部, 教授 (50072947)

Co-Investigator(Kenkyū-buntansha) KATAMINE Shigeru  Nagasaki University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (40161062)
OHSAWA Kazutaka  Nagasaki University, School of Medicine, Assistant Professor, 医学部, 助手 (90244756)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsParainfluyenza virus / Guinea pigs / Paramyxovirus / PIV3 / Experimental animals / Rats / ウイルス / マウス
Research Abstract

In order to obtain the pathological disease model in rats and guinea pigs, we attempted the infection experiments using PIV3. The animal experiments were carried out which made immune deficient state. Afterwards dynamic image of the immunological status and viral genome detection were followed after infection.
C243 strain of HPIV3 and GP8 strain of GPFV3 were inoculated i.n. to animals. The experiment animals (4 week-old male rats : SD and guinea pigs : Hartley) were administrated cyclosporin A (CsA : i.m.; 15mg/KgBW/day) daily to established immunosuppressive condition. Following results were obtained.
1. Infection experiments revealed that the virus genome could be detected in pulmonary region of guinea pigs at least day 8 through 10 post-infection, on the other hand during day 2 and 8 in immunosuppressed rats.
2. Humoral immunity conducted by IFA method was not detected during examination period in guinea pigs. Rats, however, showed IFA antibody on day 10 post-infection in spite of the treatment with cyclosporin A for immunosuppression.
3. Although the consolidation was demonstrated in guinea pig lungs, precise examination should be continuously followed.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ohsawa: "Sequence and Genetic Arrangement of the Us Region of the Monkey B Virus (Cercopithecine Herpesvirus 1) Genome and Comparison with the Us Regions of Other Primate Herpesviruses"Journal of Virology. 76・3. 1516-1520 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe: "Isolation of a Parainfluenza Virus Type 3-like Agent from Guinea Pigs"Acta Med.Nagaskiensha. 46・3-4. 15-18 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa: "Superoxide Dismutase in Senescence-accelerated Mouse Retina"The Histochemical Journal. 33. 43-50 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto: "Microbiological Contamination in Genetically Modified Animals and Proposals for a Microbiological Test Standard for National Universities in Japan"Exp.Animals. 50・5. 397-407 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 前島一叔: "実験動物感染病の対応マニュアル"アドスリー. 345 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohsawa: "Sequence and Genetic Arrangement of the Us Region of the Monkey B Virus (Cercopithecine Herpesvirus 1) Genome and Comparison with the Us Regions of Other Primate Herpesviruses"Journal of Virology. 76(3). 1516-1520 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Watanabe: "Isolation of a Parainfluenza Virus Type 3-like Agent from Guinea Pigs"Acta Medica Nagaskiensha. 46(3-4). 15-18 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa: "Superoxide Dismutase in Senescence-accelerated Mouse Retina"The Histochemical Journal. 33. 43-50 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto: "Microbiological Contamination in Genetically Modified Animals and Proposals for a Microbiological Test Standard for National Universities in Japan"Experimental Animals. 50(5). 397-407 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazutaka Ohsawa: "Genetic Characteriation of Parainfluenza Virees3"J.Vet.Ned.Sci.. 60(8). 919-922 (1998)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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