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Epidemiology on piroplasmosis of demestic animals in Mongolia

Research Project

Project/Area Number 11691167
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

IGARASHI Ikuo  Obihiro Univ., Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (80159582)

Co-Investigator(Kenkyū-buntansha) NAGASAWA Hideyuki  Obihiro Univ., Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (60172524)
FUJISAKI Kozo  Obihiro Univ., Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (00292095)
MIKAMI Takeshi  Obihiro Univ., Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (20091506)
INODUE Noboru  Obihiro Univ., Research Center for Protozoan Diseases, Assistant Professor, 原虫病研究センター, 講師 (10271751)
XUAN Xuenan  Obihiro Univ., Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10292096)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordspiroplasmosis / Mongolia / horse / cattle / antibody survey / isolation of parasites / diagnostic antigens / genetic analysis / バベシア原虫 / 組み換え抗原 / ELISA / PCR / 抗体
Research Abstract

Babesiosis in domestic animals causes enormous economic losses throughout most of the world. In Mongolia, high prevalence of babesiosis was observed in horses but not known in cattle. In the present study, in vitro cultivation was used for isolation of Babesia parasites from Mongolian horses and geneticanalysis of isolated parasites has done. Detection of antibody and DMA fragments of bovine Babesia parasites were also analyzed.
1. Antigen analysis of equine Babesia parasites and survey of antibodies
Recombinant proteins of B. equi (MEA-1) and B. caballi (BC48) were found to be useful as antigens for serodiagnostic methods such as ELISA or latex agglutination test. Both B. equi and B. caballi are endemic in horses in Central Mongolia as 81. 8% of horses were seropositive to both infections in 110 samples by IFAT. A total of 209 horse sera obtained from 2 different areas of Central Mongolia were examined by ELISA using GST-BC48. The prevalence of B. caballi infection in the 2 different ar … More eas was 90.3 % and 23.4 %.
2. Isolation of equine Babesia parasites by in vitro cultivation.
B. equi parasites were found in 33 samples from 40 blood samples. Analysis of DNA sequence of EMA-1 and EMA-2 suggests that B. equi has high genetic homology among isolates and that B. equi isolated in Mongolia has high homology in nucleotide and amino acid sequences with USDA strain.
3. Survey on ticks transmittable Babesia parasites
EMA-1 gene of B. equi and BC48 gene of B. caballi were detected in all samples and seven of the 54 samples of Dermacenor nuttalli adult ticks by PCR, respectively.
4. Survey on bovine babesiosis
Recombinant rhoptry associated protein-1 (RAP-1) of Babesia bovis was produced and i used as antigen in ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in high five insect cells might be a useful antigen for detection of antibodies to B. bovis. Less

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Hirata, H., Ikadai, H., Yokoyama, N: "Clonin of a truncated Babesia equi gene encoding a 82-kilodalton protein and itspotentia use in an enzyme-linked immunosorbent assay"J. Gun. Micorbiol. 40(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, I., Martin MD, Nagasawa, N., et al.: "Analysisi sof growth-promoting factor for Babesia caballi cultivation"J. Parasitol. 87. 1484-1486 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Battsetseg, B., Xuan, X., Jose, R., Byambaa, B., Boldbaatar D., et al.: "Diagnosis of Babesia caballi and Babesia equi infections in Dermacentor nuttalli adult"Int. J. Parasitol. 31. 384-386 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Xuan, X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I. et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"J. Clin. Micorbiol. 39. 705-709 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, H., Osorio C.R., Xuan, X., Igarashi, I., Kanemaru, et al.: "Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rohptry protein"Int. J. Parasitol. 30. 633-635 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, H., Xuan, X., Igarashi, I., et al.: "Cloning and ex ression of a 48-kDa Babesia caballi merozoite rhoptry protein and potential use o e recombinant antigen in an Enzyme-linked Immunosorbent Assay"J. Clin. Micorbiol. 37. 3475-3480 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata, H., Ikadai, H., Yokoyama, N., Xpan, X., Fujisaki , K., Suzuki, N., Mikami, T. and Igarashi, I.: "Cloning of a truncated Babesia equi gene encoding a 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay"J. Clin. Microbiol.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, I., Martin MD, Nagasawa, N., Fujisaki,K.Suzuki, N., Mikami, M., Kudo, N., Oyamada, T. and Igarashi, I.: "Analysis of growth-promoting factor for Babesia caballi cultivation"J. Parasitol.. 87. 1484-1486 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Battasetseg, B., Xpan, X., Jose, R., Byambaa, B., Boldbaastar D., Buttur, B., Battasetseg, G., Batsukh, Z., Ikadai, H., Igarashi, I., Nagasawa, H., MiKami, T. & Fujisaki, K.: "Diagnosis of Babesia caballi and Babesia equi infections in Dermacentor nuttalli adult ticks"Int. J. Parasitol.. 31. 384-386 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Xuan. X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I., et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant bacuiovirus ana evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"J. Clin. Microbiol.. 39. 705-709 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, H., Osorio C. R., Xuan, X., Igarashi, I., Kanemaru, et al.: "Detection of Babesia caballi infection by enzyme- linked immunosorbent assay using recombinant 48-kDa merozoite rohptry protein"Int. J. Parasitol.. 30. 633-635 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai, H., Xuan, A., Igarashi, I., Tanaka, S.,Kanemura, T., et al.: "Cloning and expression of a 48-kDa Bobesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an Enzyme-linked Immunosorbent Assay"J. Clin. Microbiol.. 37. 3475-3480 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikadai,H.,Osorio C.R.,Xuan,X.,Igarashi,I. et al.: "Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rohptry protein."International Journal for Parasitology. 27. 633-635 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Xuan,X.,Larsen,A.,Ikadai,H.,Tanaka,T.,Igarashi,I. et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay."J.Clin.Micorbiol.. 39. 705-709 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Xuan,X.,Igarashi,I et al.: "Detection of antibodies to Babesia equi in horses by larex agglutination test using recombinant equi merozoite antigen-1."Clin.Daignos.Lab.Immunol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kawai S.,Igarashi I.,et al.: "Ultrastructural characteristics of Babesia caballi in equine erythrocytes in vitro"Parasitology Research. 85. 794-799 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikadai H.,Xuan X.,Igarashi I.,et al.: "Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme-linked immunosorbent assay"Journal of Clinical Micorbiology. 37. 3475-3480 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanaka T.,Xuan X.,Ikadai H.,Igarashi I.et al.: "Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA"International Journal for Parasitology. 26. 1803-1808 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ikadal H.,Osorio C.R.,Xuan X.,Igarashi I.et al.: "Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein"International Journal for Parasitology. 27(in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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