| Project/Area Number |
11691200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
FUSHIYA Shinji Tohoku University, Under Graduate School of Pharmaceutical Sciences, Associate Professor, 大学院・薬学研究科, 助教授 (80108563)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIZAKI Fumihiko Tohoku Pharmaceutical University, Professor, 教授 (20158421)
TAKANO Fumihide Tohoku University, Under Graduate School of Pharmaceutical Sciences, Research Assistant, 大学院・薬学研究科, 教務職員 (20236251)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Mongolia / crude drug / hepatitis / anti-inflammation / macrophage / nitric oxide / Scizonepeta multifida / Artemisia sieversiana / 肝障害 |
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| Research Abstract |
Study on mongolian medicinal plants was carried out in chemical and pharmacological aspects. The result of this investigation is as follows :
1) At first, more than 100 Mongolian medicinal plants were collected in the north eastern area of Mongolia on July 1999 and in the north western area on July 2000.
2) As NO is one of me critical mediators in inflammation, suppression on NO production in LPS-stimulated macrophages is a useful index to evaluate anti-inflammatory activity. So, the effect of 44 Mongolian medicinal plants on the NO production was investigated. The NO production was significantly (50 %<) inhibited by methanol extracts of Scizonepeta multifida, Halenia corniculata and Artemisia sieversiana, and moderately (30-49 %) inhibited by metanol extracts of Stellera chamaejasme, Artemisia vulgaris, Geranium pratense and Leontopodium ochroleucum at a concentration of 100 μg/ml, without any cytotoxicity. Thus, these plants might be promising sources for anti-inflammatory agents. Furthermore, the extracts of Artemisia sieversiana and Scizonepeta multifida showed a concentration-dependent inhibition within the range of 10-200 μg/ml without any toxic action, indicating both plants to be specially attractive sources for anti-inflammatory agents.
3) From the methanol extract of Halenia corniculata which showed strong inhibitory action on the NO production, an active compound was isolated by a combination of chromatographic technique. And, the active compound was identified as luteolin by the ^1H- and ^<13>C-NMR spectra.
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