| Project/Area Number |
11694137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AIZAWA Mauso Tokyo Institute of Technology, Professor, 大学院生命理工学研究科, 教授 (00016742)
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| Co-Investigator(Kenkyū-buntansha) |
KOBATAKE Eiry Tokyo Institute of Technology, Associate Professor, 大学院生命理工学研究科, 助教授 (00225484)
YANAGIDA Yasuko Tokyo Institute of Technology, Associate Professor, 大学院生命理工学研究科, 講師 (10282849)
HARUYAMA Tetsuya Kyusyu Institute of Technology, Associate Professor, 大学院生命体工学研究科, 助教授 (30251656)
J.M. クーパー グラスゴー大学, 教授
A.E.G. キャス インペリアルカレッジ, 教授
COOPER J.M. University of Glasgow, Professor
CASS A.E.G. Imperial College, Professor
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | neurotransmitter / outer cell potential recording / channel gate receptor / AMP receptor / 培養神経細胞 / 微小脳 / ニューロネットワーク / マイクロファブ / マイクロファブリケーション |
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| Research Abstract |
We have developed engineered cell based outer cell potential measurement to monitor agonist-dependent cellular responses in order to monitor a function of post-synaptic receptor. Ion gate function give rise a potentiometric change on an attached electrode. As the GluR is largely expressed in insect cell and is exhibited on a cellular surface in huge population, change of outer cell potential can be obtained drastically when a ligand is Applied. Application of a natural agonist, induced a complex and robust potentiometric response in cell expressing glutamate (natural agonist) receptor. The outer cell potential profile of ligand-gated ion channel over expressed cell is affected by agonists and antagonists. Furthermore, several pharmacological agonists of the receptor also produced the potentiometric response. The observation cleary indicated that the cellular responses and engineered cells reflect post-synaptic receptor function. This novel approach should be applicatable to other ligand gated ion channel and holds promise as a cell-based biosensor for drug screening and other applications.
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