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STUDY ON THREE DIMENTIONAL CULTURE OF REPITONAL RESIDENT CELLS(AETIFICIAL PERITONEUM)

Research Project

Project/Area Number 11694181
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionUNIVERSITY OF EAST ASIA

Principal Investigator

HORIUCHI Takashi  FAC. OF ENG PROF, 工学部, 教授 (10201758)

Co-Investigator(Kenkyū-buntansha) OHTA Yuji  SOC. OF ENG, OCHANOMIZU UNIV., ASSOC PROF, 助教授 (50203807)
NAGASAWA Koichi  FAC. OF ENG, ASSOC PROF, 工学部, 助教授 (90289299)
吉田 修  東亜大学, 大学院・総合学術研究科, 教授 (70025584)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywords腹膜透析 / 腹膜硬化症 / 細胞培養 / 三次元培養 / 画像解析 / 腹膜修復 / 細胞増殖 / 細胞遊走 / サイトカイン / RT-PCR / VEGF / TGF-β1
Research Abstract

Extracellular matrices layered with collagen and hyaluronan was formed in the Transwell (Corstar ; 0.3 μm pore size) and utilized for a solute-transfer experiment. Solutes with small molecular weight, except for urea, permeate these layers depending on concentration of both collagen and hyaluronan concentrations.
In addition to solute-transfer experiments, a possible application of 3D model is a wound-healing study. To establish this experimental set-up, defoliation of mesothelial layer was made by a droplet of IN NaOH, and then microscopically observed by CCD camera. Digital images were aquired every 30 minutes until remesothelialization was completed. These date were then analysed to calculate cell size, rate of remesothelialization. 30〜4Q% increase of cell size were observed in comparison between pre and post healing. Digital video representation demonstrates cell migration prior to cell proliferation during wound healing on the monolayered culture of human peritoneal mesothelial cell.
In conclusion 3D model of the peritoneum could be used for a various type of in vitro study such as solute-transfer experiment and wound healing experiment to elucidate fibrotic process of the peritoneum due to peritoneal dialysis procedure.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] 堀内孝, その他: "培養細胞の傷害修復過程の画像解析システムの開発"腎と透析. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 堀内孝: "新しい腹膜透析技術の展開"腎と透析. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 大塚知恵, 堀内孝: "腹膜透析が腹膜に及ぼす影響"生活工学. 4(1). 12-125 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi T, et al.: "Prolonged preservation of rat peritoneal mesothelial cell (RPMC) and rat peritoneal fibroblast (RPFB)"Clin Exp Nephrol. 5(4). 257-264 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi T. et al.: "Expression of transforming growth factor (TGF-β1) mRNA and contractility of extracellular"Clin Exp Nephrol. 4. 105-113 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 堀内孝: "腹膜硬化"CTPD. 19(3). 6-7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi, T. Inoue, T. et al: "Prolonged preservation of rat peritoneal meso-thelial cell (RPMD) and rat peritoneal fibroblast (RPFB) by a glucose degradation product (GDP) depleted and neutral pH Pd solution."Clin Exp Nephrol. 5. 257-264 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi, t.Hayashi, K.et al: "Expression of trnsforming growth factor (TGH-β1) mRNA and contractility of extra-cellular matrices in three-dimensional culture of rat peritoneal fibroblasts."Clin Exp Nephrol. 4. 105-113 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi, T.Nagasawa, K. et al: "Effect of glucose degradation products (GDP) and pH in peritoneal dialysate on proliferation of rat peritoneal mesothelial cell and fibroblast"J Am Soc Nephrol. 11. 209A (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakai, A.Horiuchi, T. et al: "Peritoneal dialysis filtration (PDF) process with protein retaining dialysate for high-permeable patients"Perit Dial Int. 19(S1). 9 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuch, T.Hayashi, K. et al: "Expression of TGF-β1mRNA and contractility of extracellular matrices (EXM) in 3D culture with rat peritoneal fibroblasts."Perit Dial Int. 19(S1). 32 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Horiuchi, t: "kinetics in CAPD"Therapeutics & Engineering. 11(4). 630-633 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 堀内 孝, その他: "培養細胞の障害修復過程の画像解析システムの開発"腎と透析. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 堀内 孝: "新しい腹膜透析技術の展開"腎と透析. (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大塚 知恵, 堀内 孝, 他: "腹膜透析が腹膜に及ぼす影響"生活工学研究. 4(1). 122-125 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Horiuchi T. et al.: "Prolonged preservation of rat peritoneal mesothelral ceil(RPMC)and rat peritoneal fibroblast(RPF13)…"Clin Exp Nephnol. 5(4). 257-264 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 堀内 孝: "腹膜硬化"CTPD. 19(5). 6-7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 大塚 知恵, 堀内 孝, 他: "腹膜透析の現状と問題点"生活工学研究. 3(2). 214-217 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi HORIUCHI, et al: "Expression of transforming growth factor (TGF-β1)mRNA and contractility of extra cellular..."Clin Exp Nephrol. 4. 105-113 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大畑淳,堀内孝: "培養腹膜線維芽細胞を用いた急性傷害モデルにおけるTGF-β1とVEGF産生遺伝子の発現"人工臓器. 29(3). S61 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi HORIUCHI, et al: "Effect of glucose degradation products (GDP) and pH in peritoneal dialysate on proliferation."J Am Soc Nephrol. 11. 209A (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 堀内 孝: "CAPDのKinetics"医工学治療. 11(4). 630-634 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] HORIUCHI,T,et al: "Expression of TGF-β1 mRNA and contractility of extra cellular matrices in 3D cuture・・・"Clinical and Experimental Nephrology. 4(1). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohta Y,et al: "The atomic force microscope : a new tool for artificial organ research"J Artif Organs. 2(2). 131-134 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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