| Project/Area Number |
11694196
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKAHASHI Hideo Institute of Molecular & Cellujlar Biosciences, The University of Tokyo, Professor, 分子細胞生物学研究所, 教授 (90013333)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Katsunori Institution, Department, The University of Tokyo, Assistant Professor, 分子細胞生物学研究所, 助手 (90291335)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Arabidopsis thallana / chloroplast / sigma factor / telomerase / TERT gene / double strand breaks / nuclear gene / イネ / Ku70-Ku80 / 高等植物 / AtTERT遺伝子 / テロメラーゼ活性 / 核コードプラスチドの因子 / テロメラーゼ活性阻害 / DNAのメチル化 / 植物細胞 / タバコ培養細胞 / テロメラーゼ / S期特異的活性化 / テロメラーゼ阻害因子 / At TERT 遺伝子 / プラスチド |
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| Research Abstract |
(1)S phase-specific and auxin-enhanced activation or telomerase was demonstrated using cell-extract of cynchronized tobacco culture cells. Genes (cDNAs) for the catalytic subunits of Arabidopsis thallana and Oryza sativa (AtFERT and OsTERT) were cloned for the first time from higher plants. Highest ArTERT expression in the apical meristems was verified using northern and in situ hybridization. (2) A proteinaceous fraction having an inhibitory activity against telomerase was found in plant cell extracts. A 40 kDa protein presumably involved in the regulation of telomerase activity was identified. (3) Genes (cDNAs) for Ku70 and Ku80 homologs of Arabidopsis thaliana (AtKu70 and AtKu80) were cloned and sequenced. A heterodimer specifically formed between purified AtKu70 and AtKu80 proteins revealed sDNA-dependent ATPase and ATP-dependent DNA helicase as well as specific binding to double-stranded DNA ends. DSBs (double-strand breaks) by bleomycin or MMS treatment of cultured cells induce the expression of these genes. (4) Six genes for the nuclear-encoded plastid sigma factors of Arabidopsis thaliana (originally named SIGA〜SIGF and later renamed SIGI〜SIG6, respectively) were identified. A T-DNA inserted mutant (sig 2-1) in SIG2 (SIGB) gene having pale-green phenotype was isolated. Several plastid-encoded tRNAs including trnE-UUC indispensable for chiorophyII (tetrapyrrole) biosynthesis in plants were drastically reduced but that some tRNAs were not affected so much by the SIG2-deficiency.
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