• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

DNA Recombination of Thermophiles

Research Project

Project/Area Number 11694207
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

KURAMITSU Seiki  Graduate School of Science, Osaka Univ. Professor, 大学院・理学研究科, 教授 (60153368)

Co-Investigator(Kenkyū-buntansha) MASUI Ryoji  Graduate School of Science, Osaka Univ. Research Associate, 理学研究科, 助手 (40252580)
KATO Ryuichi  Graduate School of Science, Osaka Univ. Research Associate, 理学研究科, 助手 (50240833)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 2000: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1999: ¥4,600,000 (Direct Cost: ¥4,600,000)
Keywordshomologous recombination / DNA repair / extreme thermophile / hyperthermophile / archae / RecA / RadA / DNA / 遺伝子組換え / Thermus thermophilus HB8 / Desulfurcoccus amyloliticus
Research Abstract

In living organisms, genetic recombination is related to the DNA damage. DNA damage often arises as a result of genetic recombination, DNA replication, and other processes. In prokaryotes, about fifty enzymes participate in the DNA repair reactions. From thermophilic bacteria including hyperthermophile, we have cloned the DNA-repair related genes, which include recombinational repair enzymes (RecA, RuvA, RuvB, RuvC, ssb), base excision repair enzymes (MutM, MutY, EndoIII, EndoV), nucleotide excision repair enzymes (UvrA, UvrB, UvrC, UvrD), mismatch repair-enzymes (MutS, MutL), photolyase, and DNA polymerase I and ligase. Most of these DNA repair systems are common to many organisms, including those of Homo sapiens. These thermophilic enzymes, as expected, were stable. It was also found that the domains of the respective proteins can be easily isolated by partial digestion with protease. Some repair enzymes, including MutM, UvrB, and photolyase, have been crystallized and their three-dimensional structures have been determined. These thermophilic enzymes have been adapted to high temperatures. Some hyperthermophilic repair enzymes dramatically changed its conformation and increased the activity above 75℃

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kil,Y.V.: "Efficient Strand Transfer by the RadA Recombinase from the Hyperthermophilic Archaeon Desulfurococcus amyloliticus"J Bacteriol.. 182(1). 130-134 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Spies,M.: "The RadA Protein from a Hyperthermophilic Archaeon P. islandicum is a DNA-Dependent ATPase with a transition Temperature at 75℃"Eur.J.Biochem.. 267. 1125-1137 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isaev-Ivanov,: "Fluorescence and Excitation Escherichia coli RecA Protein Spectra Analyzed Separately for Tyrosine and Tryptophan Residues"Arch.Biochem.Biophys.. 376(1). 124-140 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugahara,M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA, MutM (Fpg), from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19(15). 3857-3869 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kil, Y.V.: "Efficient Strand Transfer by the RadA Recombinase from the Hyperthermophilic Archaeon Desulfurococcus amyloliticus"J.Bacteriol.. 182 (1). 130-134 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Spies, M.: "The RadA Protein from a Hyperthermophilic Archaeon P.islandicum is a DNA-Dependent ATPase with a transition Temperature at 75℃"Eur.J.Biochem.. 267. 1125-1137 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Isaev-Ivanov: "Fluorescence and Excitation Escherichia coli RecA Protein Spectra Analyzed Separately for Tyrosine and Tryptophan Residues"Arch.Biochem.Biophys.. 376 (1). 124-140 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sugahara, M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA, MutM (Fpg), from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19 (15). 3857-3869 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakagawa,N.: "Crystal Structure of Thermus thermophilus HB8 UvrB Protein,A Key Enzyme of Nucleotide Excision Repair"J.Biochem.. 126(6). 986-990 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kil,Y.V.: "Efficient Strand Transfer by the RadA Recombinase from the Hyperthermophilic Archaeon Desulfurococcus amyloliticus."J.Bacteriol.. 182(1). 130-134 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Spies,M.: "The RadA Protein from a Hyperthermophilic Archaeon Pyrobaculum islandicum is a DNA-Dependent ATPase that Exhibits Two Disparate Catalytic Modes,with a transition Temperature at 75℃"Eur.J.Biochem.. 267. 1125-1137 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Isaev-Ivanov,V.V.: "Fluorescence and Excitation Escherichia coli RecA Protein Spectra Analyzed Separately for Tyrosine and Tryptophan Residues"Arch.Biochem.Biophys.. 376(1). 124-140 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugahara,M.: "Crystal Structure of a Repair Enzyme of Oxidatively-Damaged-DNA,MutM (Fpg) , from an Extreme Thermophile, Thermus thermophilus HB8"EMBO J.. 19(15). 3857-3869 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yokoyama,S.: "Structural Genomics Projects in Japan"Nature Struct.Biol.. 7. 943-945 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kil,Y.V: "Efficient Strand Transfer by the RadA Recombinase from the Hyperthermophilic Archaeon Desulfurococcus amyloliticus"J.Bacteriol.. 182・1. 130-134 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato,R.: "Effect of ssDNA and Temperature on Activity of Thermus thermophilus RecA Protein"Eur.J.Biochem.. 259. 592-601 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hoseki,J.: "Directed Evolution of Thermostable Kanamycin-Resistant Gene Products in an Extremely Thermophilic Bacterium, Thermus thermophilus"J.Biochem.. 126・5. 951-956 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Spies,M: "The RadA Protein from a Hyperthermophilic Archaeon P. islandicum is a DNA-Dependent ATPase with a transition Temperature at 75℃"Eur.J.Biochem.. (印刷中). (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Isaev-Ivanv,V.V.: "Fluorescence and Excitation Escherichia coli RecA Protein Spectra Analyzed Separately for Tyrosine and Tryptophan Residues"Arch.Biochem.Biophys.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 増井 良治,倉光 成紀: "好熱菌全ゲノムの配列決定とその意義"蛋白質核酸酵素. 44・2. 165-170 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 倉光 成紀: "実験室の小さな生きものたち"羊土社. 4 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi