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Role of Sox gene family in regulation of development

Research Project

Project/Area Number 11694209
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionOsaka University

Principal Investigator

HISATO Kondoh  Osaka Univ., IMCB, Professor, 細胞生体工学センター, 教授 (70127083)

Co-Investigator(Kenkyū-buntansha) YUSUKE Kamachi  Osaka Univ., IMCB, Assistant Professor, 細胞生体工学センター, 助手 (90263334)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsSOX2 / Pax6 / Gene activation / δEF3 / Lens / Enhancer / Cell differentiation / HMG domain / パートナー因子 / HMG / SOX / ニワトリ / マウス / ヒト / zebrafish
Research Abstract

Embryonic organogenesis proceeds under the strong influence of tissue interactions involving extracellular signaling molecules. The signaling molecules, once received by a cell, activate intracellular mechanisms which lead to dynamic changes in transcriptional regulation and to a new state of cell differentiation. Thus, transcriptional regulation is the basis of cell differentiation, while tissue interactions spatially and temporally organize the events of this occurrence.
Sox transcription factors, binding to ATTGTT and related sequences, are emerging as key players of cell differentiation, among which Sox2 is probably the best studied case. Sox2 is expressed in the ICM and epiblast before gastrulation, then the primordium of CNS, sensory placodes, anterior alimentary tract, etc.
In the case of lens induction, Sox2 expression is activated in the region of head ectoderm already expressing Pax6, and this initiates lens development. We demonstrate that Sox2 and Pax6 proteins physically interact and make a ternary complex with regulatory region DNAs, and the complex strongly activates genes for lens differentiation.
Generally, Sox2 activates genes only in cooperation with partner factors which bind to an adjacent site of a regulatory region, and it pairs with different partners in different type of cells. In this way, the same Sox2 protein regulates distinct sets of genes in cell type-dependent manner.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kamachi,Y. et al.: "Mechanism of regulatory target selection by the SOX High-Mobility-Group domain proteins as revealed by comparison of SOX1/2/3 and SOX9."Mol.Cell.Biol.. 19. 107-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uchikawa,M. et al.: "Two distinct subgroups of Group B Sox genes for transcriptional activators and repressors"Mech.Dev.. 84. 103-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondoh,H.: "Transcription factors for lens development assessed in vivo."Curr.Opin.Genet.Dev.. 9. 301-308 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamachi,Y. et al.: "Pairing SOX off with partners in regulation of embryonic development."Trends Genet.. 16. 182-187 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondoh,H. et al.: "Zebrafish mutations in Gli mediated hedgehog signaling lead to lens transdifferentiation from the adenohypophysis anlage."Mech.Dev.. 96. 165-174 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamachi, Y., Cheah, K.S.E.and Kondoh, H.: "The mechanism of regulatory target selection by the SOX High-Mobility-Group domain proteins as revealed by comparison of SOX1/2/3 and SOX9."Mol.Cell.Biol.. 19. 107-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uchikawa, M., Kamachi, Y.and Kondoh, H.: "Two distinct subgroups of Group B Sox genes for transcriptional activators and repressors : their expression during embryonic organogenesis of the chichen."Mech.Dev.. 84. 103-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondoh, H.: "Transcription factors for lens development assessed in vivo"Curr.Opin.Genet.Dev.. 9. 301-308 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamachi, Y.Uchikawa, M.and Kondoh, H.: "Pairing SOX off with partners in regulation of embryonic development."Trends Genet.. 16. 182-187 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondoh, H., Uchikawa, M., Yoda, H., Takeda, H., Furutani-Seiki, M.and Karlstrom, R.O.: "Zebrafish mutations in Gli mediated hedgehog signaling lead to lens transdifferentiation from the adenohypophysis anlage."Mech.Dev.. 96. 165-174 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kamachi,Y. et al.: "Pairing SOX off with partners in regulation of embryonic development."Trends in Genetics. 16. 182-187 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kondoh,H. et al.: "Zebrafish mutations in Gli mediated hedgehog signaling lead to lens transdifferentiation from the adenohypophysis anlage."Mechnisms of Development. 96. 165-174 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kamachi, Y. et al.: "Mechanism of regulatory target selection by the SOX High-Mobility-Group domain proteins as revealed by comparison of SOX1/2/3 and SOX9"Mol. Cell. Biol.. 19. 107-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchikawa, M. et al.: "Two distinct subgroups of Group B Sox genes for transcriptional activators and repressors : their expression during embryonic organogenesis of the chicken"Mech. Dev.. 84. 103-120 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kondoh, H.: "Transcription factors for lens development assessed in vivo"Curr. Opn. Genet. Dev.. 9. 301-308 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kamachi, Y. et al.: "Pairing SOX off with partners in regulation of embryonic development"Trends Genet.. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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