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Characterization of different enzymes catalyzing oxidative deamination of amines

Research Project

Project/Area Number 11694212
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionYamaguchi University

Principal Investigator

ADACHI Osao  Faculty of Agr., Yamaguchi U., Prof., 農学部, 教授 (20027189)

Co-Investigator(Kenkyū-buntansha) TANIZAWA Katsuyuki  Inst. Sci. Ind. Res., Osaka U., Prof., 産業科学研究所, 教授 (20133134)
TOYAMA Hirohide  Faculty of Agr., Yamaguchi U., As/Prof., 農学部, 助教授 (60240884)
MATSUSHITA Kazunobu  Faculty of Agr., Yamaguchi U., Prof., 農学部, 教授 (50107736)
HIROTA Shun  Fac. Sci., Nagoya U., As/Prof, 理学部, 助手 (90283457)
HITORSU Ken  Fac. Sci., Osaka City U., Prof., 理学部, 教授 (10047269)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordsamine oxidase / amine dehydrogenase / quinone cofactors / quinoproteins / アミン類の酸化 / カルボニル補欠分子族 / TPQ / TTQ
Research Abstract

The crystal structure of a quinohemoprotein amine dehydrogenase from Pseudomonas putida has been determined at .1.9A resolution. The enzyme comprises three non-identical subunits ; a four-domain A-subunit that harbors adi-heme cytochrome c, a seven-bladed B-propeller B-subunit that provides part of the active site, and a small G-subunit that contains a novel cross-linked. proteinous quinone cofactor, cysteine tryptophylquinone. More suprisingly, the catalytic G-subunit contains three additional chemical cross-links that encage the cysteine trytpphylquinone cofactor, involving a cysteine side chain bridged to either an Asp or Glu residue all in a hitherto unknown thicether bonding with a methylene carbon atom of acidic amino acid side chains. Thus, the structure of the 79-residue G-subunit is quite unusual, containing four internal cross-links in such a short polypeptide chain that would otherwise be difficult to fold into a globular structure.
Spermidine dehydrogenase from Citrobacter freundii and histamine dehydrogenase from Nocardioides simplex have also been crystallized for X-ray analysis, which may develop another new fields of quinoprotein chemistry. Furthermore, it is very much promising to have been succeeded in purification of cytokinin oxidase and amino-aldehyde dehydrogenase from plants sources.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] I.Vanoenberghe et al.: "The covalent structure of the amall subunit from P.putida amine dehydrogenase reveals the presence of three-novel types of internal cross-linkages"J.Biol.Chem.. 276. 42923-41931 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Datta et al.: "Structure of quinohemoporotein amine dehydrogenase with uncommon redox cofactor and highly unusual crosslinking"Proc.Natl.Acad.Sci.USA. 98. 14268-14273 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Hirota et al.: "Observation of intermediates formed during the oxidative half-reaction of copper/topa quinone-phenethylamine oxidase"Biochemistry. 40. 15789-15796 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] P.Galuszka et al.: "Cytokinin oxidase or dehydrogenase?"Eur.J.Biochem.. 268. 1-13 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Matsushita et al.: "Quinoprotein : structure, function, and biotechnological applications"Appl.Microbiol.Biotechnol.. 57. 1-19 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A.Satoh et al.: "Crystal structure of quinohemoprotein amine dehydro-qenase from Pseudomonas putida"J.Biol.Chem.. 277. 2830-2834 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Vandenberghe J, K. Kim, B. Devreese, A. Hacisalihoglu, H. Iwabuki, T. Okajima, S. Kuroda, O. Adachi J, A. Jongejan, J. A. Duine, K. Tanizawa, and J. Van Beeumen: "The Covalent Structure of the Small Subunit from Pseudomonas putida Amine Dehydrogenase Reveals the Presence of Three Novel Types of Internal Cross-Linkages, All Involving Cysteine in a Thio-Ether Bond"J. Biol. Chem. 276. 42923-42931 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Datta, Y. Mori, K. Takagi, K. Kawaguchi, Z. W. Chen, T. Okajima, S. Kuroda, T. Ikeda, K. Kano, K. Tanizawa, and F. S. Mathews: "Structure of a Quinohemoprotein Amine Dehydrogenase with an Uncommon Redox Cofactor and Highly Unusual Crosslinking"Proc. Natl. Acad. Sci. U.S.A. 98. 14268-14273 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Hirota, T. Iwamoto, S. Kishishita, T. Okajima, O. Yamauchi, K. Tanizawa: "Spectroscopic Observation of Intermediates Formed during the Oxidative Half-Reaction of Copper/Topa Quinone-Containing Phenylethylamine Oxidase"Biochemistry. 40. 15789-15796 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] P. Galuszka, I. Frebort, M. Sebela, P. Sauer, S. Jacobsen, and P. Pec: "Cytokinin oxidase or dehydrogenase ? Mechanism of cytokinin degradation in cereals"Eur. J. Biochem. 268. 1-13 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Matsushita, H. Toyama, M. Yamada & O. Adachi: "Quinoproteins : structure, function, and biotechnological applications"Appl. Microbiol. Biotechnol. 57. 1-19 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsuko Satoh, Jong-Keun Kim, Ikuko Miyahara, Bart Devreese, Isabel Vandenberghe, Ayse Hacisalihoglu, Toshihide Okajima, Shun'ichi Kuroda, Osao Adachi, Johannis A Duinel, Jozef Van Beeumen, Katsuyuki Tanizawa, and Ken Hirotsu: "Crystal Structure of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida"J. Biol. Chem. 277 (4). 2830-2834 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] I.Vancenberghe et al.: "The covalent structure of the small subunit from P. putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages"J.Biol.Chem.. 176. 42923-42931 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Datta et al.: "Structure of quinohemoporotein amine dehydrogenase with uncommon redox cofactor and highly unusual crosslinking"Proc.Natl.Acad.Sci.USA. 98. 14268-14273 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Hirota et al.: "Observation of intermediates formed during the oxidative half-reaction of copper/topa quinone-phenethylamine oxidase"Biochemistry. 40. 15789-15796 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] P.Galuszka et al.: "Cytokinin oxidase or dehydrogenase?"Eur.J.Biochem.. 268. 1-13 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Matsushita et al.: "Quinoprotein : structure, function, and biotechnological applications"Appl.Microbiol. Biotechnol.. 57. 1-19 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Satoh et al.: "Crystal structure of quinohemoprotein amine dehydrogenase from Pseudomonas putida"J.Biol.Chem.. 277. 2830-2834 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ivo Frebort 他4名: "Cellular localization and metabolic function of n-butylamine-induced amine oxidases in the fungus Aspergillus niger AKU 3302."Arch.Microbiol.. 173. 358-365 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Niculescu Mihaela 他5名: "Redox hydrogel-based amperometric bienzyme electrodes for fish freshness monitoring."Anal.Chem.. 72. 1591-1597 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Marek Sebela 他6名: "Characterization of a homogenous plant aminoaldehyde dehydrogenase."Biochim.Biophys.Acta. 1480. 329-341 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Marek Sebela 他5名: "A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines."Arch.Biochem.Biophys.. 384. 88-89 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Marek Sebela 他5名: "FAD-containing polyamine oxidases : a timely challenge for researchers in biochemistry and physiology of plants."Plant Sci.. 160. 197-207 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S. Hirota et al.: "Spectroscopic characterization of carbon monoxide complexes generated for cu/TOPA amine oxidases"Biocehm.. 38・43. 14256-14263 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.. Frebort et al.: "Localization of quinoprotein amine oxidase of Aspergillus niger"Arch. Microbiology. (掲載受理済). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] A. Hacisalihoglu et al.: "Spectroscopic and chemical characterization of amine dehydrogenase of Pseudomonas putida"Eur. J. Biochem.. (掲載受理済). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 足立 収生: "タンパク質化学・酵素・第4巻"廣川書店. 5 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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