| Project/Area Number |
11694231
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
KOHGO Yutaka ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (10133183)
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| Co-Investigator(Kenkyū-buntansha) |
ASHIDA Toshifumi ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (50261409)
AYABE Tokiyuoshi ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, INSTRUCTOR, 医学部, 助手 (90301019)
FUJIMOTO Yoshinori ASAHIKAWA MEDICAL COLLEGE, SCHOOL OF MEDICINE, P, INSTRUCTOR, 医学部, 助手 (90292127)
奥村 利勝 旭川医科大学, 医学部, 講師 (60281903)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | INTERNATIONAL SCIENCE EXCHANGE / ANTIMICROBIAL PEPTIDE / a-DEFENSIN / PANETH CELL / INFLAMMATORY BOWEL DISEASE / defensin / 自然免疫 / 粘膜防御 / 内因性抗菌ペプチド / paneth cell defensin / NRAMP / IL-18R / innate immunity |
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| Research Abstract |
In this study we analyzed the expression of antimicrobial peptide in IBD intestine and colon by biological and immunological technique. We have found that 1) As well as a mouse intestine reported before, isolated crypts from normal human intestine could exocrine the antimicrobial peptide by the LPS stimulation, ex vivo. 2) In situ hybridization and RT-PCR of isolated human crypts revealed that antiomicrobial activity was caused by human a-defensin (HD-5, HD-6), released from Paneth cells. 3) Isolated human crypts from IBD intestine also produce the antimicrobial peptide as well as that of normal, noninflammed intestine. 4) Paneth cells in UC colon (metaplastic Paneth cell) also release the antimicrobial activity. 5) Metaplastic Paneth cells in human stomach also have and release antimicrobial activity that kills effectively H. Pylori.
These results were submitted to publication.
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