| Project/Area Number |
11694233
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
IGARASHI Kazuhiko DEPT.BIOCHEMISTRY, HIROSHIMA UNIVERSITY, PROFESSOR, 医学部, 教授 (00250738)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Akira DEPT.BIOCHEMISTRY, TOHOKU UNIVERSITY, SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 大学院・医学系研究科, 助手 (50292214)
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| Project Period (FY) |
2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | LCR / chromatin / transcription factor / BTB domain / POZ domain / knockout mice / インスレーター / 細胞分化 / Maf |
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| Research Abstract |
This study focused on the tissu-and cell lineage-specific regulation of chromatin structures in hematopoietic cells. There are several transcription factors that bind to the locus control region of hematopoietic-specific genes. To understand their roles, we generated mutant mice lacking the transcription factor genes bach1 or bach2. Further analysis of the phenotypic changes of these mice may pave a way to understand functional link among regulation of transcription, chromatin structures, and cell differentiation. Along these experiments, we also screened for proteins that interact with Bach1 or Bach2 using yeast two hybrid screen. Isolated candidates include proteins that regulate actin structure, transcritional co-regulators, and structural proteins, suggesting their implication in regulation of chromatin structures.
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