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Drosophila Neuromuscular Junction Formation in Culture

Research Project

Project/Area Number 11694242
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionGunma University School of Medicine

Principal Investigator

KIDOKORO Yoshiaki  Gunma University School of Medicine Institute for Behavioral Sciences, Division of Physiology Professor, 医学部, 教授 (00053083)

Co-Investigator(Kenkyū-buntansha) KUROMI Hiroshi  Gunma University School of Medicine Institute for Behavioral Sciences, Division of Physiology Associate Professor, 医学部, 助教授 (30009633)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsCultered neurons / Drosophila / mutants / synaptic transmission / shibire / neuromuscular junctions / vesicle recycling / synaptic vesicle pools
Research Abstract

i)Cultures of embryonic Drosophila neurons
Eggs(3 to 4 hours after fertilization)were collected and sterilized with hypochlorate. The content was sucked into a glass pipette and transferred into Schneider's medium supplemented with fetal calf serum and insulin. Neurons send out processed within a few days and form contacts each other. Co-cultures with muscle cells are now being tried.
ii)Size of vesicle pools, rates of mobilization, and recycling at neuromuscular synapses of a Drosophila mutant, shibire
Two vesicle pools, readily releasable(RRP)and reserve(RP)pools, are present at Drosophila neuromuscular junctions. Using a temperature-sensitive mutant, shibir^<ets>, we studied pool sizes and vesicle mobilization rates. In shibire^<ts>, due to lack of endocytosis at nonpermissive temperatures, synaptic currents continuously declined during tetanic stimulation until they ceased as the result of vesicle depletion. By then, approximately 84,000 quanta were relased. Vesicles were mobilized from RP at a rate of 1/7-1/10 of RRP.Cytochalasin D inhibited mobilization of vesicles from RP, allowing us to estimate the size of RRP as 14%-19% of all vesicles. Vesicle recycling supports synaptic transmission during prolonged tetanic stimulation and the maximum recycling rate was 1000 vesicles/s.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kuromi, H., Kidokoro, Y.: "Tetanic stimulation recruits vesicles from reserve pool via a cAMP-mediated process."Neuron. 27. 133-143 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Delgado, R., Kidokoro, Y.Labarca, P.: "Vesicle recycling and synaptic transmission during prolonged synaptic activity in larval Drosophila motor..."Journal of Physiology. 523P. 67S (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihara, M., Suzuki, K., Kidokoro, Y.: "Two independent pathways mediated by cAMP/PKA enhance spontaneous trasmitter release at Drosophila..."Journal of Neuroscience. 20. 8315-8322 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Delgado, R., Maureira, C., Oliva, C., Kidokoro, Y...: "Size of vesicle pools, rates of mobilization and recycling at the larval neuromuscular junction of a temperature..."Neuron. 28. 941-953 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kidokoro, Y.: "Synapse Formation."Encyclopedia of Life Sciences 2000. 90. 1691-1697 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishikawa K, Kidokoro Y: "Halothane presynaptically depresses synaptic transmission in wild-type Drosophila larvae but not in halothane..."Anesthesiology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishikawa K, Kidokoro Y: "Octopamine inhibits synaptic transmission at the larval neuromuscular junction in Drosophila melanogaster."Brain Research. 837. 67-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshihara M, Ueda A, Zhang D, & Kidokoro, Y.: "Selective effects of neuronal-synaptobrevin mutations on transmitter release evoked by sustained."Journal of Neuroscience. 19. 2432-2441 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Delgado, R., Kidokoro, Y.and Labarca, P.: "Vesicle recycling and synaptic transmission during prolonged synaptic activity in larval Drosophila motor end-plates"J Physiol. 523P. 59P (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Delgado R, Maureira C, Oliva C, Kidokoro Y.and Labarca P.: "Size of vesicle pools, rates of mobilization and recycling at the larval neuromuscular junction of a temperature-sensitive Drosophila mutant, shibire"Neuron. 28. 941-953 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kuromi,H.,Kidokoro,Y.: "Tetanic stimulation recruits vesicles from reserve pool via a cAMP-mediated process."Neuron. 27. 133-143 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Delgado,R.,Kidokoro,Y.,Labarca,P.: "Vesicle recycling and synaptic transmission during prolonged synaptic activity in larval Drosophila motor..."Journal of Physiology. 523P. pp.67S (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshihara,M.,Suzuki,K.,Kidokoro,Y.: "Two independent pathways mediated by cAMP/PKA enhance spontaneous transmitter release at Drosophila..."Journal of Neuroscience. 20. 8315-8322 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Delgado,R.,Maureira,C.,Oliva,C.,Kidokoro,Y...: "Size of vesicle pools, rates of mobilization and recycling at the larval neuromuscular junction of a temperature..."Neuron. 28. 941-953 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kidokoro,Y.: "Synapse Formation."Encyclopedia of Life Sciences 2000. 90. 1691-1697 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishikawa K,Kidokoro Y: "Halothane presynaptically depresses synaptic transmission in wild-type Drosophila larvae but not in halothane-...."Anesthesiology.

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishikawa K,Kidokoro Y: "Octopamine inhibits synaptic transmission at the larval neuromuscular junction in Drosophila melanogaster."Brain Research. 837. 67-74 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshihara M,Ueda A,Zhang D,...& Kidokoro.Y.: "Selective effects of neuronal-synaptobrevin mutations on transmitter release evoked by sustained..."Journal of Neuroscience. 19. 2432-2441 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kidokoro,Y.: "Synapse formation"Encyclopedia of Life Sciences. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishikawa K,Kidokoro Y: "Halothane presynaptically depresses synaptic transmission in wild-type Drosophila larvae but not in halothane-"Anesthesiology. 90. 1691 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshihara M,Ueda A,Zhang D,...& Kidokoro,Y.: "Selective effects of neuronal-synaptobrevin mutations on transmitter release evoked by sustained"Journal of Neuroscience. 19. 2432-2441 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kuromi H,Kidokoro Y: "The optically determined size of exo/endo cycling vesicle pool correlates with the quantal content"Journal of Neuroscience. 19. 1557-1565 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhang D,kuromi H,Kidokoro Y: "Activation of metabotropic glutamate receptors enhances svnaptic transmission at the Drosophila"Neuropharmcology. 38. 645-657 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishikawa K,Kidokoro Y: "Octopamine inhibits synaptic transmission at the larval neuromuscular junction in Drosophila melanogaster"Brain Research. 837. 67-74 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Rheuben MB,Yoshihara M.,Kidokoro Y.: ""International Review of Neurobiology Volume 43-Neuromuscular Junctions in Drosophila""Academic Press. (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Zhang D,Kuromi H,,Kidokoro Y: ""SlowSynapticResponsesandModulation""Springer,Tokyo. 455 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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