| Project/Area Number |
11694251
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tokyo |
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Principal Investigator |
SEYAMA Yousuke University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (90010082)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIJIMA Yasunobu University of Tokyo, Graduate School of Medicine, Associate, 大学院・医学系研究科, 助手 (90272426)
SATOH Yoichi Iwate Medical University, School of Medicine, Professor, 医学部, 教授 (40118253)
KUBOTA Shunichiro University of Tokyo, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00260480)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Harderian gland / golden hamster / fatty acid synthesis / androgen / nuclear receptor / acyl-CoA dehydrogenase |
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| Research Abstract |
The Harderian gland of golden hamster secretes copious lipids consisting mainly of 1-alkyl-2, 3-diacylglycerol(ADG), the composition of which shows marked sexual differences. Male gland contains straight chain fatty acids, while female gland additionally contains branched chain ones. We found these differences were caused by the androgenic regulation of acyl-CoA dehydrogenases which are the key enzymes for the formation of branched chain fatty acids. The sexual differences were detectable by analyzing ADG on TLC.Namely, ADG profile was composed of 3 spots in male while 2 spots in female. Utilizing the characteristics of this gland, we monitored the effects of androgenic and anti-androgenic materials by analyzing ADG profile of secreted fluid from the gland. Castrated male hamsters(2 weeks after operation)were subcutaneously injected with testosterone with or without anti-androgens such as flutamide. We obtained about 1 μ1 of gland fluid with a glass capillary from each animal for 2 weeks. ADG profile on TLC changed into female type by castration. Testosterone treatment returned he profile to male type time- and dose -dependently. However these changes were abolished when anti-androgens were additionally injected. The inhibition was also dependent on the dose of anti-androgens. These results clearly show that we can easily evaluate the effects of androgenic and anti-androgenic materials using animals noninvasively.
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