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NMR-spectrophotometer integrated device to monitor neuronal activity

Research Project

Project/Area Number 11694262
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionShiga University of Medical Science

Principal Investigator

INUBUSHI Toshiro  Shiga University of Medical Science, Molecular Neuroscience Research Center, Professor, 分子神経科学研究センター, 教授 (20213142)

Co-Investigator(Kenkyū-buntansha) SHIINO Akihiko  Shiga University of Medical Science, Department of Medicine, Assistant Professor, 医学部, 講師 (50215935)
MORIKAWA Shigehiro  Shiga University of Medical Science, Molecular Neuroscience Research Center, Associate Professor, 分子神経科学研究センター, 助教授 (60220042)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsin vivo NMR / MR spectroscopy / metabolic activity / non-invasive / brain / near infra red spectroscopy / visible spectroscopy / blood oxygen level / NMR / 生体NMR / 脳循環動態 / MR代謝画像 / 脳代謝機能 / 代謝動態 / 酸素運動 / 近赤外分光 / 可視分光
Research Abstract

1. Fast scanning echo planar imaging (EPI) approach in MRI was incorporated into newly developed highly sensitive ^1H-detected in vivo ^<13>C NMR spectroscopy, which was developed in our laboratory by the integration of hetero nuclear multiple quantum coherence (HMQC) technique and non-invasive localization method.
2. Injected glucose labeled by ^<13>C into living organs was traced non-invasively by ^1H-detected ^<13>C NMR method. Glucose reached to the highest level in 0.5 hours and decreased rapidly after a bolas injection. However, glutamine/glutamate derived by the metabolic reaction of the glucose reached to the highest level in 1 hour and stayed at this high level for approximately two more hours. In this stable period a chemical shift imaging technique was applied to investigate the distribution of these metabolites in brain.
3. A visible spectrophotometer has been developed to investigate blood oxygen level in living animals. In order to gain sensitivity for blood-oxygen level detection, we employed a Soret-band wavelengths of oxy and deoxy hemoglobin and isosbestic point of these two absorbances. Another feature of the photometer is that this instrument has independent two detectors, which can simultaneously measure right and left hemisphere in rat brain.
4. Near infra red spectroscopy was employed to monitor non-invasively oxygen level in blood. For this a spectrophotometer having 750 and 830nm light sources and 13-channel detector was developed in Dr. Chance's laboratory. With this instrument deoxygenation and blood volume were traced during brain ischemia in rat and their maps were also constructed in approximately 30 seconds.
5. A partially liganded NO-hemoglobin was elucidated to bear unique nature in oxygen binding. It was found to be in typical T-structure by spectroscopic and oxygen binding studies. Therefore, this type of hemoglobin may be utilized as an effective oxygen release at the oxygen-demanded tissues or organs in-living system.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Suzuki E. et al: "^1H-and ^<31>P-magnetic resonance spectroscopy and imaging as a new diagnostic tool to evaluate neuropathic in Type II diabetic"Diabetologia. 43(2). 165-172 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murashita J. et al: "Altered brain energy metabolism in lithium-resistant bipolar disorder detected by photic stimulated ^<31>P-MR spectroscopy."Psychol Med.. 30(1). 107-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki E. et al: "Usefulness of waveform analysis of popliteal artery type II diabetic patients using gated magnetic resonance 2D-cine-PC imaging..."Diabetologia.. 43. 1031-1038 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morikawa S. et al: "Fast ^<13>C-Glucose Metabolite Mapping in Rat Brain Using ^1H Echo Planar Spectroscopic Imaging Technique at 2T."J Magn Reson Imaging. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiguchi I., et al: "Brain proton magnetic resonance spectroscopy and brain atrophy in myotonic dystrophy."Arch.Neurol.. 56. 325-330 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morikawa S, et al: "Effects of blood sugar level on rat transient focal brain ischemia consecutively observed by diffusion weighted EPI and ^1H echo"Magn Reson Med. 42. 895-902 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morikawa S et al.: "Gluconeogenesis and phosphoenergetics in rat liver during endotoxemia."J.Surg.Res.. 74. 179-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Arai T et al.: "Measurement of local cerebral blood flow by magnetic resonance imaging : in vivo autoradiographic strategy using ^<17>O-labeled water.."Brain Res.Bull.. 45. 451-456 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishida T, et al.: "A primitive pathway of porphyrin biosynthesis and enzymology in Desulfovibrio vulgaris."Proc.Natl.Acad.Sci.USA. 95. 4853-4858 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okada M, et al.: "Influence of aging or left ventricular hypertrophy on the human heart : contents of phosphorus metabolites measured by ^<31>P MRS."Magn Reson.Med.. 38. 772-782 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakajima M, et al.: "Estimation of volume doubling time and cell loss in an experimental rat glioma model in vivo."Acta Neurochir (Wien). 140. 607-613 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi K, et al.: "Hepatic energy metabolism during ketamine and isoflurane anesthesia in haemorrhagic shock."Br.J.Anaesth.. 80. 782-787 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato T, et al.: "Magnetic resonance spectroscopy in affective disorders."J.Neuropsychiatry Clin.Neurosci.. 10(2). 133-47 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato, T et al.: "Photic stimulation-induced alteration of brain energy metabolism measured by ^<31>P-MR spectroscopy in patients with MELAS."J Neurol Sci.. 155(2). 182-5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ishii H, et al.: "Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses."J.Cereb.Blood Flow Metab.. 18. 931-934 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato T, et al.: "Decreased brain intracellular pH measured by 31P-MRS in bipolar disorder : a confirmation in drug-free patients and correlation with white matter hyperintensity."Eur Arch Psychiatry Clin Neurosci. 248. 301-306 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nishino H et al.: "Estrogen protects against while testosterone exacerbates vulnerability of the lateral striatal artery to chemical hypoxia by 3-nitropropionic acid."Neurosci Res. 30. 303-312 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakasu Y, et al.: "Lactate and NAA mapping assessed by proton NMR chemical-shift imaging in seizures."Epilepsia. 39(supple 5). 69-70 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiino A, et al.: "Poor recovery of mitochondrial redox state in CA1 after transient forebrain ischemia in gerbils."Stroke. 29. 2421-2425 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaoka A, et al.: "Renal ischemia/reperfusion remotely improves myocardial energy metabolism during myocardial ischemia via adenosine receptors in rabbits : effects of "remote preconditioning"."J.Am.Coll.Cardiol.. 33. 556-564 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murashita J, et al.: "Age-dependent alteration of metabolic response to photic stimulation in the human brain measured by ^<31>P MR-spectroscopy."Brain Res.. 818. 72-76 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akiguchi I, et al.: "Brain proton magnetic resonance spectroscopy and brain atrophy in myotonic dystrophy."Arch.Neurol.. 56. 325-330 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morikawa S, et al.: "Effects of blood sugar level on rat transient focal brain ischemia consecutively observed by diffusion-weighted EPI and ^1H echo planar spectroscopic imaging."Magn Reson Med. 42. 895-902 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiino A, et al.: "Noninvasive evaluation of the malignant potential of intracranial meningiomas performed using proton magnetic resonance spectroscopy."J.Neurosurg.. 91. 928-934 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kato T, et al.: "Relationship of energy metabolism detected by ^<31>P-MRS in the human brain with mental fatigue."Neuropsychobiology.. 39(4). 214-8 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hamakawa H, et al.: "Quantitative proton magnetic resonance spectroscopy of the bilateral frontal lobes in patients with bipolar disorder."Psychol Med.. 29. 639-44 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiino A, et al.: "Three-dimensional redox image of the normal gerbil brain."Neuroscience. 91. 1581-1585 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki E, et al.: "^1H- and ^<31>P-magnetic resonance spectroscopy and imaging as a new diagnostic tool to evaluate neuropathic foot ulcers in Type II diabetic patients."Diabetologia. 43(2). 165-72 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murashita J, et al.: "Altered brain energy metabolism in lithium-resistant bipolar disorder detected by photic stimulated ^<31>P-MR spectroscopy."Psychol Med.. 30(1). 107-15 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morikawa Set al.: "^<13>C-Glucose Metabolite Mapping in Rat Brain Using ^1H Echo Planar Spectroscopic Imaging Technique at 2T."J Magn Reson Imaging. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki E. et al: "^1H- and ^<31>P-magnetic resonance spectroscopy and imaging as a new diagnostic tool to evaluate neuropathic in Type II diabetic"Diabetologia. 43(2). 165-172 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Murashita J. et al: "Altered brain energy metabolism in lithium-resistant bipolar disorder detected by photic stimulated ^<31>P-MR spectroscopy."Psychol Med.. 30(1). 107-115 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki E. et al: "Usefulness of waveform analysis of popliteal artery type II diabetic patients using gated magnetic resonance 2D-cine-PC"Diabetologia.. 43. 1031-1038 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Morikawa S.and Inubushi T.: "Fast ^<13>C-Glucose Metabolite Mapping in Rat Brain Using ^1H Echo Planar Spectroscopic Imaging Technique at 2T."J Magn Reson Imaging. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 来見良誠 他: "MRガイド下肝腫瘍穿刺術におけるスペーサーの有用性"肝臓. 41. 507-508 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuyama K. et al.: "Myocardial high-energy phosphates hepatic redox state in..."J.Surg.Res.. 82. 88-94 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takaoka A. et al.: "Renal ischemia/reperfusion remotely improves myocardial..."J.A.Coll.Cardiol.. 33. 556-564 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Murashita J. et al.: "Age-dependent alteration of metabolic response to photic..."Brain Res.. 818. 72-86 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akiguchi I. et al.: "Brain proton magnetic resonance spectroscopy and brain..."Arch.Neurol.. 56. 325-330 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kato T. et al.: "Relationship of energy metabolism detected by 31P MRS in the..."Neuropsychobiology. 39. 214-218 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hamakawa H.: "Quantitative proton magnetic resonance spectroscopy of the..."Psychol.Med.. 29. 639-644 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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