| Project/Area Number |
11694274
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
神経・脳内生理学
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| Research Institution | Osaka University |
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Principal Investigator |
FUKUDA Yutaka Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (90028598)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tetsu Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (60263282)
SAWAI Hajime Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20202103)
WATANABE Masami Institute for Development Research, Department of Physiology, Principal Investigator, 主任研究員 (10093486)
MIYOSHI Tomomitsu Osaka University, Graduate School of Medicine, Assistan Professor, 医学系研究科, 助手 (70314309)
SASAKI Hitoshi Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40104236)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥19,740,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2001: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2000: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1999: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | optic nerve / regeneration / retina / ganglion cell / axon / receptive field / functional evaluation / behavior |
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| Research Abstract |
1. Facilitatory effects of intraocularly applied neurotrophins on survival and axonal regeneration of cat retina ganglion cells (Watanabe et al., 2002) : After intraocular applications of a mixture of BDNF, CNTF and forskolin, not only the survival but also the axonal regeneration by peripheral nerve graft was significantly enhanced in axotomized cat retinal ganglion cells. The effect was especially conspicous on beta cells.
2. Capacities of visual information processing in axotomized cat retinal ganglion cells (Takao et al., 2002) : 5 and 14 days after optic nerve transection visual function of axotomized cat retina ganglion cells were evaluated. Most of the surviving cells revealed normal visual responses which enabled to classify into Y or X cells. However, the size of the receptive field center was shrunken in some of the axotomized cells.
3. Quantitative evaluations of survival and axonal regeneration of retinal ganglion cells in bcl-2 overexpressed mice (Inouet et al., 2002) : In b
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cl-2 transgenic mice the survival of axotomzied ganglion cells was significantly higher than in wild type mice, whereas the axonal regeneration, assessed by peripheral nerve transplanation, was similar between the two. This result appears to be in conflict with the well known suggestion that bcl-2 has promoting effect on axonal regeneration of retinal ganglion cells as well as their survival.
4. Survival promoting effects of electrical stimulation to the cut optic nerve on axotomized rertinal ganglion cells in rats (Morimoto et al., 2002) : Monophasic electrical plulses (50 μA, 50μs, At s , 20 Hz) applied for 2 hrs to the cut end of the optic nerve enhanced the one-week survival of retinal gangion cells up to 83 % from the control level (53 %).
5. Behavioral evaluations of functional recovery of vision (Sasaki et al., 1999) : Hamsters having a reconstructed retino-collicular projection with peripheral nerve graft revealed visually guided behaviors when assessed by three different behavioral paradigms ; open field behavior, classical aversive conditioning and operant avoidance learning.
6. Review works on degeneration, surival and axonal regeneration of cat retinal ganglion cells (Watanabe and Fukuda, 2002) : After summarizing our recent works on cat retinal ganglion cells, we reviewed the present status of the worldwide trials towards the protection of damaged retinal ganglion cells and the promotion of their axonal regeneration in adult mammals. Less
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