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Neuron specific calcium-binding Protein, calbrain, is involved in memory formation.

Research Project

Project/Area Number 11694284
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKAGAWA MEDICAL UNIVERSITY

Principal Investigator

TOKUDA Masaaki  Kagawa Medical University, Physiology, Professor, 医学部, 教授 (10163974)

Co-Investigator(Kenkyū-buntansha) OKABE Akinobu  Kagawa Medical University, Microbiology, Professor, 医学部, 教授 (20093677)
SUGIMOTO Katsuyoshi  Kagawa Medical University, Physiology, Research Associate, 医学部, 助手 (80322270)
KOBAYASHI Ryoji  Kagawa Medical University, Chemistry, Professor, 医学部, 教授 (00020917)
TAKEUCHI Yoshiki  Kagawa Medical University, Chemistry, Professor, 医学部, 教授 (20116619)
山口 文徳  香川医科大学, 医学部, 助手 (40271085)
松下 治  香川医科大学, 医学部, 助教授 (00209537)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordscalbrain / CaM kinase II / LTP / S100 / brain / hypothermia / NMDA / alcoholism / カルシウム結合蛋白質 / 記憶 / Ca^<2+> / calmodulin-dependent protein kinase II / calcineurin / NMR
Research Abstract

A novel Ca2+-binding protein, termed calbrain, was isolated from a human brain cDNA library. The analysis of deduced amino acid sequence revealed that calbrain contains two putative EF-hand motifs that show significantly high homology to those of the calmodulin(CaM)family rather than two EF-hand protein families. Studies in vitro revealed that calbrain competitively inhibited CaM binding to Ca2+/calmodulin-dependent kinase II(Ki = 129 nM)and reduced its kinase activily and autophosphorylation.
Ca2+/calmodulin-dependent protein kinase I(CaM-kinase I)in rat retina was shown to be present in rat retina. Developmental studies revealed that CaM-kinase I expression increased in accordance with postnatal development. Expression of CaM-kinase I in the retinas of rats raised in the complete darkness markedly decreased.
CaMK-I was shown to mainly localize in the cytoplasm of the control and LTP-induced neurons, and a significant increase of immunoreactivity was observed in the latter neurons. A part of CaMK-I was found to translocate to the nuclei of LTP-induced hippocampal CA1 neurons.
To investigate the mechanism of chronic cell death following postischemic hypothermia, the change of N-methyl-D-aspartate receptor(NMDAR)was examined in the CA1 subfield of the gerbil hippocampus. At 1 week following postischemic hypothermia(32 degrees Cx4 h), all CA1 neurons survived ; however, immunoreactivity of NMDAR1 increased in neuronal perikarya whereas decreased in dendrites in the CA1 neurons. LTP was also significantly depressed at 1 week after hypothermia.
Morphological changes of the suprachiasmatic nucleus(SCN)of the hypothalamus were investigated in mice exhibiting intoxication signs of stages 2 or 3 after a short application term of 6% ethanol. Alterations in glial cells and neurons were examined using anti-glial fibrillary acidic protein(GFAP)and short-term ethanol exposure led to strong expression of GFAP-immunoreactivily(GFAP-IR)in the dorsomedial part of the SCN.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Yamashita K: "Purification of bovine S100A12 from recombinant Escherichia coli."Protein Expr Purif. 16・1. 47-52 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi K: "Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain."J Biol Chem. 274・6. 3610-3616 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsumura T: "The expression of Ca^<2+>/calmodulin-dependent protein kinase I in rat retina is regulatory light stimulation"Vision Res. 39・19. 3165-3173 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ahmed BY: "Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation."Neurosci Lett. 290・2. 149-153 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyamoto O: "Depression of long term potentiation in gerbil hippocampus following postischemic hypothermia."Brain Res. 873・1. 168-172 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoriotomo I: "Short-term ethanol exposure alters calbindin D28k and glial fibrillary acidic protein immunoreactivity in hippocampus of mice."Brain Res. 879・1-2. 55-64 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamashita K et al.: "Purification of bovine S100A12 from recombinant Escherichia coli."Protein Expr Purif. 16-1. 47-52 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi K et al.: "Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulindependent protein kinase II activity in the brain."J Biol Chem. 274-6. 3610-3616 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsumura T et al.: "The expression of Ca^<2+>/calmodulin-dependent protein kinase I in rat retina is regulated by light stimulation."Vision Res. 39-19. 3165-3173 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ahmed BY et al.: "Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I, -II and -IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation."Neurosci Lett. 290-2. 149-153 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyamoto O et al.: "Depression of long term potentiation in gerbil hippocampus following postischemic hypothermia."Brain Res. 873-1. 168-172 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoriotomo I et al.: "Short-term ethanol exposure alters calbindin D28k and glial fibrillary acidic protein immunoreactivity in hippocampus of mice."Brain Res. 879-1/2. 55-64 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tamura N: "Enhanced expression and activation of Ca(2+)/calmodulin-dependent protein kinase IV in hepatocellular carcinoma."Cancer. 89・9. 1910-1916 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ahmed BY: "Expression and subcellular localization of multifunctional calmodulin-dependent protein kinases-I,-II and-IV are altered in rat hippocampal CA1 neurons after induction of long-term potentiation."Neurosci Lett. 290・2. 149-153 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyamoto O: "Depression of long term potentiation in gerbil hippocampus following postischemic hypothermia."Brain Res. 873・1. 168-172 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsushita O: "Substrate recognition by the collagen-binding domain of clostridium histolyticum class I collagenase."J Biol Chem. in press. (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Satoriotomo I: "Short-term ethanol exposure alters calbindin D28k and glial fibrillary acidic protein immunoreactivity in hippocampus of mice."Brain Res. 879・1-2. 55-64 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamashita K: "Purification of bovine S100A12from recombinant Escherichia coli."Protein Expr Purif. 16・1. 47-52 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi K: "Calbrain,a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain."J Biol Chem. 274・6. 3610-3616 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tsumura T: "The expression of Ca^<2+>calmodulin-dependent protein kinase I in rat retina is regulated by light stimulation."Vision Res. 39・19. 3165-3173 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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