| Project/Area Number |
11694285
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Ehime University |
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Principal Investigator |
MIKI Tetsuro Ehime University Faculty of Medicine Professor, 医学部, 教授 (00174003)
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| Co-Investigator(Kenkyū-buntansha) |
IGASE Michiya Ehime University Faculty of Medicine University Hospital Instructor, 医学部・附属病院, 助手 (90314955)
NAKURA Jun Ehime University Faculty of Medicine University Hospital Assistant Professor, 医学部・附属病院, 講師 (70304607)
KOHARA Katsuhiko Ehime University Faculty of Medicine Associate Professor, 医学部, 助教授 (30260384)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | SNP (single nucletoide polymorphism) / polymorphic DNA maker / multifuctorial diseases / hypertension / 症例一対照研究 |
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| Research Abstract |
We have introduced a SNP (single nucleotide polymorphism) as a polymorphic DNA maker in order to analyze the multifuctorial diseases. An analysis of 10 polymorphisms spanning 26 kb of the ACE (angiotensin converting enzyme) gene revealed a limited number of haplotypes in Caucasian British families due to strong linkage disequilibrium. It was reported that a haplotype tree (cladogram) was constructed with three main branches (clades A-C) which account for 90% of the observed haplotypes. Clade C is most likely derives from clades A and B following an ancestral recombination event. We examined 97 Japanese samples and made haplotypes to see the difference between British and Japanese, using HPLO (Hawley, ME.and Kidd, KK. : 1995). There are two haplotypes (TATATTGIA3 : 43%, CCCTCCADG2 : 28%) whose frequency is over 5 percent. There are no haplotype which belongs to clade C.We confirmed the linkage disequilibrium in the ACE gene and found the unique genetic founder effect in Japan. This findings may lead to the new consideration of global genetic drift. There was no significant difference on haplotype frequency between normal healthy controls and patients with hypertension.
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