| Project/Area Number |
11694306
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osaka City University Graduate School of Medicine |
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Principal Investigator |
TANAKA Akemi Osaka City University Graduate School of Medicine, Department of Pediatrics, Associate Professor, 医学部, 助教授 (30145776)
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| Co-Investigator(Kenkyū-buntansha) |
FUKAI Kzuyoshi Osaka City University Graduate School of Medicine, Department of Dermatology, Associate Professor, 医学部, 助教授 (20244642)
OTANI Shuzo Osaka City University Graduate School of Medicine, The 2nd Department of Biochemistry, Professor, 医学部, 教授 (80047068)
SATO Makoto Fukui Medical School, The 2nd Department of Anatomy, Professor, 医学部, 教授 (10222019)
MAEDA Mitsuyo Osaka City University Graduate School of Medicine, The 1st Department of Anatomy, Lecturere, 医学部, 講師 (40122080)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2000: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | MICROGLIA CELL / ADENOVIRUS VECTOR / LYSOSOMAL STORAGE DISEASE / β-GLUCURONIDASE DEFICIENCY / GENE EXPRESSION / GFP |
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| Research Abstract |
Gene delivery into the brain via blood vessels is quite difficult because of the blood-brain-barrier (BBB). It is speculated that microglia cells would go through BBB.We established a strain of cultured microglia cell from newborn mice brain for the vehicle of gene into the brain. The cells were labeled with a reporter gene of GFP and injected into the left ventricle of heart of Sly mice. Sly mouse, the deficiency of β-glucuronidase, is a mouse model for human disease of mucopolys accharidosis type VII, which is a systemic disorder including the brain caused by the accumulation of glycosaminoglycans. It is suggested that delivery of the deficient enzyme or the gene into each organ would improve the disease.
In the literature, a number of experiments of gene therapy for the brain have been done, but none of them has not been successful. In our study, the cultured microglia cells could enter the brain tissue via blood vessel only when BBB was injured by the disease progression, but they do not go through normal BBB.
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