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Molecular approaches to the genetic diversity of malaria parasites

Research Project

Project/Area Number 11694323
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionOsaka Institute of Tecnology

Principal Investigator

TANABE Kazuyuki  Osaka Institute of Technology, Lab. of Biology, Professor, 工学部, 教授 (40047410)

Co-Investigator(Kenkyū-buntansha) SAITO Atsuko (ITO Atsuko)  Kobe University School of Medicine, Associate Professor, 医学部, 助教授 (00223131)
KIMURA Masatsugu  Osaka City Univ. Medical School, Res. Assoc., 医学部, 助手 (60195378)
KANABARA Hiroji  Nagasaki Univ. Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (20029789)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2001: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsMalaria / Plasmodium / Gene / Antigen / MSP1 / PGR / Recombination / Polymorphism / 多様性 / オーシスト
Research Abstract

The genetic diversity of surface antigens of malaria parasites was studied on field isolates from geographic endemic areas. Analysis of about 500 isolates by PCR and DNA sequencing revealed the followings.
1. Polymorphism of the merozoite surface antigen gene (PfMsp-1) of P. falciparum: Linkage disequilibrium between polymorphic sites in the 5'-region (blocks 2-6) and 3'-region (block 17) of PfMsp-1 in parasite population from Thailand, Vietnam and Brazil The strength of this linkage disequilibrium correlated with the intensity of transmission of malaria. It is suggested that both selection and genetic drift are involved in this linkage disequilibrium. The diversity of PfMsp-1 was very limited in Vanuatu with very strong linkage disequilibrium between the 5'- and 3' - polymorphic sites. Extremely low rate of mixed clone infections in Vanuatu suggests that the rate of recombination in PfMsp-1 depends on not only the intensity of transmission but the frequency of mixed infection and the number of alleles present in an endemic area.
2. Polymorphism of the merozoite surface antigen gene (PvMsp-1) of P. vivax: In order to examine in-depth structural organization of PvMsp-1 , complete nucleotide sequencing of the gene of 40 isolates obtained from geographic areas was done. Sequence alignment revealed that (i) PvMsp-1 consist of 7 variable blocks interspersed with 8 conserved blocks, (ii) variation is primarily dimorphic, and (iii) potential recombination sites occur throughout the gene. These results suggest that allelic recombination is the major mechanism for generating the diversity in PvMsp-1.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] L.A.Da Silva, et al.: "Allelic diversity and antibody recognition of Plasmodium falciparum merozoite surface protein-1 under hypoendemic malaria transmission in the Brazilian Amazon"Infection and Immunity. 67. 5906-5916 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M.Kimura, et al.: "Gametocyte-dominant expression of a novel type ATPase in Plasmodiumyoelii"Mol.Biochem.Parasitol.. 104. 331-336 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Tanabe, et al.: "Selection and genetic drift of polymorphisms within the merozoite surface protein-1 gene of Plasmodium falciparum"Gene. 241. 325-331 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Takeo, et al.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein(Fp) and iron-sulfur(Ip) subunits"Mol.Biochem.Parasitol.. 107. 191-205 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.He, et al.: "Plasmodium falciparum : effective use of the CO_2-NaHCO_3 buffere system for evaluating chloroquine resistance"Exp.Parasitol.. 94. 121-124 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D.J.Conway, et al.: "A principal target of human immunity to malaria identified by molecular population genetic and immunological analyses"Nature Medicine. 6. 689-692 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C.Putaporntip, et al.: "Intragenic recombination in the 3'portion of the merozoite surface protein 1 gene of Plasmodium vivax"Mol.Biochem.Parasitol.. 109. 111-120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J.Fu, et al.: "Epitope-specific impairment of antibody production to MSP1 of Plasmodium falciparum in symptomatic patients with malaria"Parasitol.Res.. 86. 345-351 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Sakihama, et al.: "Long PCR amplification of Plasmodium falciparum DNA extracted from filter paper blots"Exp.Parasitol.. 97. 50-54 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C.Putaporntip, et al.: "Diversity in the thrombospondin-relaed adhesive protein gene(TRAP) of Plasmodium vivax"Gene. 268. 97-104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] L.A.Da Silveira, et al.: "Sequence diversity and linkage disequilibrium within the merozoite surface protein-1(Msp-1)locus of Plasmodium falciparum : a longitudinal study in Brazi"J.Eukary.Biol.. 48. 433-439 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Sakihama, et al.: "Limited recombination events in merozoite surface protein-1 alleles of Plasmodium falciparum on islands"Gene. 279. 41-48 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田辺和裄: "マラリア原虫表面抗原の分子生物学『日本における寄生虫の進歩』"目黒寄生虫館. 672 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田辺和裄, 他: "『マラリア学ラボマニュアル』"菜根出版. 290 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] L. A. Da Sllva, et al.: "Allelic diversity arid antibody recognition of Plasmodium falciparum merozoite surface protein-1 under hypoendemic malaria transmission in the Brazilian Amazon"Infection and Immunity. 67. 5906-5916 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Kimura, et al.: "Gametocyte-dominant expression of a novel type ATPase in Plasm odium yoelii"Mol. Biochem., Parasitol.. 104. 331-336 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Tanabe, et al.: "Selection and genetic drift of polymorphisms within the merozoite surface protein-1 gene of Plasmodium falciparum"Gene. 241. 325-331 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Takeo, A. et al.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria: Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein (Fp) and iron-sulfur (Ip) subunits"Mol. Biochem., Parasitol.. 107. 191-205 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. He. et al.: "Plasmodium falciparum: effective use of the CO_2-NaHCO_3 buffere system for evaluating chloroquine resistance"Exp. Parasltol.. 94. 121-124 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] D. J. Conway, et al.: "A principal target of human Immunity to malaria Identified by molecular population genetic and immunological analyses"Nature Medicine. 6. 689-692 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C. Putaporntip, et al.: "Intragenic recombination in the 3' portion of the merozoite surface protein 1 gene of Poasmodium vivax"Mol. Biochem. Parastitol.. 109. 111-120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J. Fu, M. et al.: "Epitope-specific impairment of antibody production to MSP1 of Plasmodium falciparum in symptomatic patients with malaria"Parasitol. Res.. 86. 345-351 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Sakihama, et al.: "Long PCR amplification of Plasmodium falciparum DNA extracted from filter paper blots"Exp. Parasltol.. 97. 50-54 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] C. Putaporntip, et al.: "Diversity in the thrombospondin-relaed adhesive protein gene (TRAP) of Plasmodium vivax"Gene. 268. 97-104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] L. A. Da Silveira, et al.: "Sequence diversity arid linkage disequilibrium within the merozoite surface protein-1 (Msp-1) locus of Plasmodium falciparum: a longitudinal study in Brazil"J. Eukarv. Blol.. 48. 433-439 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Sakihama, et al.: "Limited recombination events in merozoite surface protein-1 alleles of Plasmodium falciparum on islands"Gene. 279. 41-48 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Sakihama, A.Kaneko, T.Hattori, K.Tanabe: "Limited recombination events in merozoite surface protein-I alleles of Plasmodium falciparum on islands"Gene. 279. 41-48 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] L.A.Da Silveira, K Tanabe et al.: "Sequence diversity and linkage disequilibrium within the merozoite surface protein-1 (Msp-1) locus of Plasmodium falciparum: a longitudinal study in Brazil"Journal of Eukaryotic Microbiology. 48. 433-439 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] C.Putaporntip, K.Tanabe et al.: "Diversity in the thrombospondin-relaed adhesive protein gene (TRAP) of Plasmodium vivax"Gene. 268. 97-104 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田辺和桁, 先濱直子: "マラリア原虫の抗原多型と集団生物学"蛋白質・核酸・酵素. 47. 5-12 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 田辺和桁: "マラリア原虫の遺伝と集団生物学"遺伝. 55. 48-52 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Sakihama,T.Mitamura K.Tanabe et al.: "Long PCR amplification of Plasmodium falciparum DNA extracted from filter paper blots"Experimental Parasitology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] C.Putaporntip,S.Jongwutiwes H.Kanbara and K.Tanabe: "Intragenic recombination in the 3' portion of the merozoite surface protein 1 gene of Plasmodium vivax"Molecular and Biochemical Parasitology. 109. 111-120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Conway,D.R.Cavaaugh,K.Tanabe et al: "A principal target of human immunity to malaria identified by moelcular population genetic and immunological analyses"Nature Medicine. 6. 689-692 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田辺和裄,先濱直子 ら: "熱帯熱マラリア原虫表面抗原Msp-1多型における選択と遺伝的浮動"Parasitology Internatinal. 49. 49 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Matsuo,N.Sakihama K.Tanabe and M.Kawabata: "Diversity of merozoite surface protein-1 (MSP-1) in the Solomon Iskands"Parasitology Internatinal. 49. 90 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 田辺和裄: "マラリア原虫のゲノムと抗原"Progress in Medicine. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 田辺和裄 ら: "マラリア学ラボマニュアル"菜根出版. 290 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] S.He,A.Saito-Ito,K.Tanabe et al.: "Plasmodium falciparum:effective use of the CO_2-NaHCO_3 buffere system for evaluating chloroquine resistance"Experimental Parasitology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Fu,M.Hato,K.Tanabe et al.: "Epitope-specific impairment of antibody production to MSP1 of Plasmodium falciparum in symptomatic patients with malaria"Parasitology Research. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Takeo,K.Kita,K.Tanabe et al: "Succinate dehydrogenase in Plasmodium falciparum mitochondria:Molecular characterization of the SDHA and SDHB genes for the catalytic subunits"Molecular and Biochemical Parasitology. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Tanabe,M.Kimura et al: "Selection and genetic drift of polymorphisms within the merozoite surface protein-1 gene of Plasmodium falciparum"Gene. 241. 325-331 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Kimura,K.Tanabe,et al: "Gametocyte-dominant expression of a novel type ATPase in Plasmodium yoelii"Molecular and Biochemical Parasitology. 104. 331-336 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] L.A.Da Silva,K.Tanabe,et al: "Allelic diversity and antibody revognition of Plasmodium falciparum merozoite surface protein-1 under hypoendemic malaria transmission in the Brazilian Amazon"Infection and Immunity. 67. 5906-5916 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2021-11-25  

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