• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation method development of endocrine disruptor effect and pollution analysis by its application

Research Project

Project/Area Number 11793003
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field 分離・精製・検出法
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

TODA Kei  Kumamoto University, Faculty of Science, Associate professor, 理学部, 助教授 (90264275)

Co-Investigator(Kenkyū-buntansha) NAKONO Naoko  Kumamoto Immuno Research Lab, Chief Researcher, 開発部, 主任研究員
NAKATA Haruhiko  Kumamoto University, Graduate school of Science and Technology, Research Associate, 大学院・自然科学研究科, 助手 (60311875)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsendocrine disruptors / immunoassay / electrochemical sensor / polychlorinated biphenyls / 抗原抗体 / PCB
Research Abstract

Some kinds of devices have been developed to determine specific chemicals electrochemically after immunoreactions. In the one of the devices, a SPR cell was used as a reaction field. Immobilization of antibody and immunoreactions were conducted with monitoring with the SPR signal and final product by enzyme reactions were detected electrochemically. This method had an advantage that the complicated procedure of sandwich immunoassay process could be monitored and could be successfully continued to the final electrochemical enzyme assay. The antigen could be measured by the SPR signal only when the concentration was high, and the electrochemical determination had 1000 time greater sensitivity than the SPR's. A reaction field was fabricated on a gold film by the process establish with the help of the SPR. Usually, the immobilization needs long time such as one night, when the antibody was conuected on a glass substrate with glutalaldehyde. On the contrary, the antibody could be immobilized on the gold film in a short time. When we used SPR instrument, the immobilization area was limited. But, by using the large immobilization area, the enzyme reaction could be done without the flow stop for the incubation. The other device consisted of a micro-capillary. With this capillary device, only 5 μL of sample was needed for the determination. The detection limit of IgG was very small, 2 amol. This device was expected to be used with inserting into a living body directly.
PCBs contained in living things and sediments were investigated by means of GC/MS. It was found that the PCB level at the bottom was higher than the mouth of the bay. PCBs levels in many samples of B. pectinirostris were measured. The concentration was related to the body length rather than to their age.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] K.Toda: "A wall-jet ring disk electrode fabricated within a thin-layered micromachined cell"Journal of Electroanalytical Chemistry. 479. 57-63 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 戸田 敬: "液的マイクロリング電極による電気化学的ガス計測方法の検討"分析化学. 49. 989-995 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Toda: "Fluorometric field instrument for continuous measurement of atmospheric hydrogen sulfide"Analytical Chemistry. 73. 5716-5724 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Nakata: "Growth-dependent and species-specific accumulation of polychlorinated biphenyls(PCBs) in tidal flat organisms collected from the ariake sea, Japan"Archives of Environmental Contamination and Toxicology. 42. 222-228 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Nakata: "Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs"Environmental Pollution. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中田晴彦: "有明海の干潟底質におけるPCBs濃度分布とその残留特性"環境化学. 12(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Toda, S. Oguni, Y. Takamatsu and I. Sanemasa: "A wall-jet ring disk electrode fabricated within a thin-layered micromachined cell"Journal of Electroanalytical Chemistry. 479. 57-63 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Toda, M. Kitazaki and I. Sanemasa: "Amperometric gas detection using a micro-ring electrode coupled with a gas-absorbing droplet"Bunsekl Kagaku. 49. 989-995 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Toda, P. K. Dasgupta, J. Li, G. A. Tarver and G. M. Zarus: "Fluorometric field instrument for continuous measurement of atmospheric hydrogen sulfide"Analytical Chemistry. 73. 5716-5724 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Nakata, Y. Sakai and T. Miyawaki: "Growth-dependent and species-specific accumulation of polychlorinated biphenyls (PCBs) in tidal flat organisms collected from the Ariake Sea, Japan"Arch. Environ. Contam. Toxicol.. 42. 222-228 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Nakata, A. Sakakibara, M. Kanoh, S. Kudo, H. Watanabe, N. Nagai, N. Miyazaki, Y. Asano and S. Tanabe: "Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and ncn-ortho coplanar PCBs"Environmental Pollution. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H Nakata T. Miyawaki and Y. Sakai: "Concentrations and isomer compositions of polychlorinated biphenyls (PCBs) in tidal flat sediments from the Ariake Sea and their implications for sources and distribution"Kankyo Kagaku. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Nakata: "Growth-dependent and species-specific accumulation of polychlorinated biphenyls(PCBs) in tidal flat organisms collected from the ariake sea, Japan"Arch. Environ. Contain. Toxicol.. 42. 222-228 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Nakata: "Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs"Environmental Pollution. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中田晴彦: "有明海の干潟底質におけるPCBs濃度分布とその残留特性"環境化学. 12(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Minh: "Isomer-specific accumulation and toxic assessment of polychlorinated biphenyls including coplanar congenters in cetaceans from the North Pacific and Asian coastal waters"Arch.Environ. Contam. Toxicol.. 39. 398-410 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] I.Monirith: "Organochlorine contamination in fish and mussels from Cambosia and other Asian countries"Water Science and Technology. 42. 241-252 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] D.Muir: "Circumpolar trends of PCBs and organochlorine pesticides in the Arctic marine environment inferred from levels in ringed seal"Environ. Sci.Technol.. 34. 2431-2438 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Nakata: "Population strtucture in minke whale from the North Pacific examined by persistent organic pollutants as chemical tracers"IWC Scientific Committee Workshop to review the Japanese whale research program under special permit for North Pacific mink whales. 1-9 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi