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Practical development of the lipid vesicle as surfactant assemblies having a functional molecular-recognition -toward to a cancer therapy in DDS-

Research Project

Project/Area Number 11793006
Research Category

Grant-in-Aid for University and Society Collaboration

Allocation TypeSingle-year Grants
Research Field 反応・分離工学
Research InstitutionEhime University

Principal Investigator

KATO Keichi  EHIME University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (10117088)

Co-Investigator(Kenkyū-buntansha) HEIKE Yuji  Inst. Center of Shikoku Cancer-Therapy, Chief. Dr., ガン遺伝子研究室, 室長
KAWAKUBO Akihiro  Yamaki Co. Ltd, Reseach Dev., ChiefLeader, 研究開発室, 主任
SUGAHARA Takuya  Faculty of Agriculture Ehume Univ., Ins cractor, 農学部, 助手 (00263963)
SUZUKI Yoji  School of Medicine, Ehime Univ. Instructor., 医学部, 助手 (20226567)
TATEISHI Norihiko  School of Medicine, Ehime Univ, Associate Professor, 医学部, 助教授 (90236555)
佐伯 俊昭  国立病院四国がんセンターガン遺伝子研究室, 室長
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2001: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsDDS / Cancer Therapy / Gene transfection / Lipid vesicle / Liposome / Immunovesicle / Alga lectin / Hybridoma / 糖鎖 / ハイブリドーマ
Research Abstract

The main purpose of this work is the new preparation of the lipid vesicles, which have a function of specific targeting to a cancer cell to apply to a cancer therapy in DDS : the vesicles are mainly composed of Span80 (sorbitan monooleate) and the stability is very excellent.
First, as a targeting ligand to a cancer cell as "missile device" which was immobilized to the vesicle, new alga lectin was chosen. The alga lectin was extracted and purified from a red alga, Eucheuma serra. The lectin was abbreviated as ESA (Eucheuma serra agglutinin). The ESA associates with high-mannose type carbohydrate chain specifically. The ESA-imoobilized vesicle (abbreviated as ESA-vesicle) was found to specifically combine with cancer cells, such as human colon adenocarcinoma (colo201) and not to combine to MCF10-2A and Fibroblast as a normal cell lines. The ESA had an excellent heat-stability and the inhibition of the growth of a cancer cell. Thus, ESA can be applied to both the targeting ligand and canc … More er cell or the inhibitor of cancer-cell growth. The growth inhibition was also found to induce an apoptosis. The ESA-vesicle flew smoothly in the superior artery of rabbit.
The in-vivo experiments on the injection of the ESA-vesicle into rats suggested no cytotoxic effect of the vesicle on the rats because of the rat's living during 5 weeks without decrease in the weight. Moreover, in the in-vivo experiments on the injection of the ESA-vesicle into the nude mouse carrying colo201, the growth inhibition of the colo201 was aiso observed. Thus, the ESA-vesicle is expected to using for the cancer therapy in DDS.
Next, some kinds of antibodies as a "missile device" as mentioned above were also immobilized on the Span80 vesicle. The specifical combines of the immunovesicles with each targeting cell were successfully confirmed the tageting experiments in vitro. The transfection of the DNA or prasmid entrapped in the immunovesicles to the targeting cells were also confirmed.
Thus, the above ESA-vesicles as well as an immunovesicle were expected to be applied to either DDS or gene transfection. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] T.Sugahara, Y.Ohama, A.Fukuda, M.Hayashi, A.Kawakubo, K.Kato: "The Cytotoxic effect of Eucheuma serra agglutinin(ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles"Cytotechnonogy. 36. 93-99 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 加藤敬一, 菅原卓也, 佐々木毅, 丸山祐樹, 吉村和憲, 立石憲彦, 川久保明宏: "癌細胞への標的機能を有する脂質ベシクルの創製"化学工学シンポジウムシリーズ. 76. 230-238 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 加藤敬一, 川嶋真一, 菅原卓也: "癌治療DDSへの利用を目指した脂質ベシクルの創製 -抗体を固定化したイムノベシクル-"ケミカルエンジニアリング. 45、6. 49-55 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Kato, T.Sugahara, S.Kawashima, Y.Hayashi, A.Oda, Y.Hisada, T.Hayashiari, T.Sasaki: "Preparation of an immunovesicle for specific combining to target cells toward either DDS or gene transfection"Proc.of Biotechnology 2000(The World Congress on Biotechnology). 2. 79-81 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Sugahara, K.Kato, A.Fukuda, A.Yoshihiro, N.Yoshimura, N.Tateishi, A.Kawakubo: "Application of the lipid vesicle immobilized Eucheuma serra lectin for target-specific drug delivery"Proc.of Biotechnology 2000(The World Congress on Biotechnology). 2. 83-85 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 加藤敬一: "W/O/Wエマルションを用いた血中抗体濃度の測定"ケミカルエンジニアリング. 44. 53-59 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Kato, T.Sugahara, S.Kawashima, Y.Hayashi, A.Yoshihiro, T.Sasaki: "Study of the specific binding between lipid vesicles and human-human hybridoma toward either DDS or gene transfection"Animal Cell Technology, Products from Cells, Cells as Products, Kluwer Academic Publishers. 429-431 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K.Kato, T.Sugahara, Y.Maruyama, N.Yoshimura, N.Tateishi, Y.Suzuki, A.Kawakubo, T.Sasaki: "Study of DDS for a cancer therapy applying lipid vesicle immobilizing Eucheuma Serra Lectin"Animal Cell Technology, Products from Cells, Cells as Products, Kluwer Academic Publishers. 433-435 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 加藤 敬一: "微粒子工学大系 第II巻応用技術 「癌治療」"フジ・テクノシステム. 6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sugahara. Y. O. hama. A. Fnkuda, M. Hayashi, A. Kawakubo and K. Kato: "The Cytotoxic effect of Eucheuma serra agglutinin (ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles"Cytotechnonogy. 36. 93-99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kato, T. Sugahara, T. Sasaki, Y. Maruyama, N. Yoshimura, A. Yoshihiro, N. Tateishi, A. Kawakubo and T. Saeki: "New preparation of lipid vesicle having a targeting function of to cancer cell"Chemical Engineering Symposium Series,. 76. 230-238 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keiichi Kato, Shinnichi Kawashima and T. Sugahra: "New preparation of lipid vesicle toward to a cancer therapy ―IgG-immobilizied Immunovesicle ―"Chemical Engineering. 45, No. 6. 49-55 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kato, T. Sugahara, S. Kawashima, Y. Hayashi, A. Oda, Y. Hisaeda T. Hayashi and T. Sasaki: "Preparation of an immunovesicle for specific combining to target cells toward either DDS or gene transfection"Proc. of Biotechnology 2000 (The World Congress on Biotechnology). 2. 79-81 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sugahara, K. Kato, A. Fnkuda, A. Yoshihiro, N. Yohimura, N. Tateishi and A. Kawakubo: "Application of the lipid vesicle immobilized Eucheuma serra lectin for target-specific drug delivery"Proc. of Biotechnology 2000 (The World Congress on Biotechnology). 2. 83-85 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keiichi Kato: "The measurement of the concentration of antibody in blood"Chemical Engineering.. 44. 53-59 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kato, T. Sugahara, S. Kawashima, Y. Hayashi, A. Yoshihiro and T. Sasaki: "Study of the specific binding between lipid vesicles and human-human hybridoma toward either DDS or gene transfection"Animal Cell technology, Products from Cells, Cells as Products. Kluwer Academic Publishers. 429-431 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Kato,T. Sugahara,Y. Maruyama,N. Yoshimura,N. Tateishi,Y. Suzuki, A. Kawakubo and T. Sasaki: "Study of DDS for a cancer therapy applying lipid vesicle immobilizingEucheuma Serra Lectin"Animal Cell Technology, Products from Cells, Cells as Products, Kluwer Academic Publishers. 433-435 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keiichi Kato: "Biryuushi kougaku taikei No. 2, Ohyou gijyutsu (Cancer therapy)"Fuji Techno. System. 694-699 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Sugahara, Y.Ohama, A.Fukuda, M.Hayashi, A.Kawakubo, K.Kato: "The Cytotoxic effect of Eucheuma serra agglutinin(ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles"Cytotechnonogy. 36. 93-99 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤 敬一, 菅原卓也, 佐々木毅, 丸山祐樹, 吉村和憲, 立石憲彦, 川久保明宏: "癌細胞への標的機能を有する脂質ベシクルの創製"化学工学シンポジウムシリーズ. 76. 230-238 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤敬一, 川嶋真一, 菅原卓也: "癌治療DDSへの利用を目指した脂質ベシクルの創製-抗体を固定化したイムノベシクル-"ケミカルエンジニアリング. 45、6. 49-55 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Kato, T.sugahara, S.Kawashima, Y.Hayashi, A.Oda, Y.Hisaeda, T.Hayashi, T.Sasaki: "Preparation of an immunovesicle for specific combining to target cells toward either DDS or gene transfection"Proc. of Biotechnology 2000 (The World Congress on Biotechnology). 2. 79-81 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Sugahara, K.Kato, A.Fukuda, A.Yoshihiro, N.Yohimura, N.Tateishi, A.Kawakubo: "Application of the lipid vesicle immobilized Eucheuma serra lectin for target-specific drug derivery"Proc. of Biotechnology 2000 (The World Congress on Biotechnology). 2. 83-85 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤敬一: "W/O/Wエマルションを用いた血中抗体濃度の測定"ケミカルエンジニアリング. 44. 53-59 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Kato, T.Sugahara, S.Kawashima, Y.Hayashi, A.Yoshihiro, T.Sasaki: "Study of the specific binding between lipid vesicles and human-human hybridoma toward either DDS or gene transfection"Animal Cell Technology, Products from Cells, Cells as Products. Kluwer Academic Publishers,. 429-431 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.kato, T.Sugahara, Y.Maruyama, N.Yoshimura, N.Tateishi, Y.Suzuki, A.Kawakubo, T.Sasaki: "Study of DDS for a cancer therapy applying lipid vesicle immobilizing Eucheuma Serra Lectin"Animal Cell Technology, Products from Cells, Cells as Products Kluwer Academic Publishers,. 433-435 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤敬一: "微粒子工学大系 第II巻応用技術「癌治療」"フジ・テクノシステム. 6 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 加藤敬一,川嶋真一,菅原卓也: "癌治療DDSへの利用を目指した脂質ベシクルの創製-抗体を固定化したイムノベシクル-"ケミカルエンジニアリング. 45、6. 49-55 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Kato,T.Sugahara,S.Kawashima,Y.Hayashi,A.Oda,Y.Hisaeda,T.Hayashi and T.Sasaki: "Preparation of an immunovesicle for specific combining to target cells toward either DDS or gene transfection"Proc.of Biotechnology 2000 (The World Congress on Biotechnology). 2. 79-81 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Sugahara,K.Kato,A.Fukuda,A.Yoshihiro,N.Yohimura,N.Tateishi and A.Kawakubo: "Application of the lipid vesicle immobilized Eucheuma serra lectin for target-specific drug derivery"Proc.of Biotechnology 2000 (The World Congress on Biotechnology). 2. 83-85 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 加藤敬一: "微粒子ハンドブック第II巻「癌治療」"(株)フジ・テクノシステム(2001年10月出版予定). 6

    • Related Report
      2000 Annual Research Report
  • [Publications] 加藤敬一: "W/O/Wエマルションを用いた血中抗体濃度の測定"ケミカルエンジニアリング. 44. 53-59 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Kato, T.Sugahara, S.Kawashima, Y.Hayashi, A.Yoshihiro and T.Sasaki: "Study of the specific binding between lipid vesicles and human-human hybridoma toward either DDS or gene transfection"Animal Cell Technology, Products from Cells, Cells as Products . Kluwer Academic Publishers. 429-431 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Kato, T.Sugahara, Y.Maruyama, N.Yoshimura, N.Tate-ishi, Y.Suzuki, A.kawakubo and T.Sasaki: "Study of DDS for a cancer therapy applying lipid vesicle immobilizing Eucheuma Serra Lectin"Animal Cell Technology, Products from Cells, Cells as Products . Kluwer Academic Publishers. 433-435 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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