| Project/Area Number |
11793006
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| Research Category |
Grant-in-Aid for University and Society Collaboration
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| Allocation Type | Single-year Grants |
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| Research Field |
反応・分離工学
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| Research Institution | Ehime University |
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Principal Investigator |
KATO Keichi EHIME University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (10117088)
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| Co-Investigator(Kenkyū-buntansha) |
HEIKE Yuji Inst. Center of Shikoku Cancer-Therapy, Chief. Dr., ガン遺伝子研究室, 室長
KAWAKUBO Akihiro Yamaki Co. Ltd, Reseach Dev., ChiefLeader, 研究開発室, 主任
SUGAHARA Takuya Faculty of Agriculture Ehume Univ., Ins cractor, 農学部, 助手 (00263963)
SUZUKI Yoji School of Medicine, Ehime Univ. Instructor., 医学部, 助手 (20226567)
TATEISHI Norihiko School of Medicine, Ehime Univ, Associate Professor, 医学部, 助教授 (90236555)
佐伯 俊昭 国立病院四国がんセンターガン遺伝子研究室, 室長
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 2001: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | DDS / Cancer Therapy / Gene transfection / Lipid vesicle / Liposome / Immunovesicle / Alga lectin / Hybridoma / 糖鎖 / ハイブリドーマ |
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| Research Abstract |
The main purpose of this work is the new preparation of the lipid vesicles, which have a function of specific targeting to a cancer cell to apply to a cancer therapy in DDS : the vesicles are mainly composed of Span80 (sorbitan monooleate) and the stability is very excellent.
First, as a targeting ligand to a cancer cell as "missile device" which was immobilized to the vesicle, new alga lectin was chosen. The alga lectin was extracted and purified from a red alga, Eucheuma serra. The lectin was abbreviated as ESA (Eucheuma serra agglutinin). The ESA associates with high-mannose type carbohydrate chain specifically. The ESA-imoobilized vesicle (abbreviated as ESA-vesicle) was found to specifically combine with cancer cells, such as human colon adenocarcinoma (colo201) and not to combine to MCF10-2A and Fibroblast as a normal cell lines. The ESA had an excellent heat-stability and the inhibition of the growth of a cancer cell. Thus, ESA can be applied to both the targeting ligand and canc
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er cell or the inhibitor of cancer-cell growth. The growth inhibition was also found to induce an apoptosis. The ESA-vesicle flew smoothly in the superior artery of rabbit.
The in-vivo experiments on the injection of the ESA-vesicle into rats suggested no cytotoxic effect of the vesicle on the rats because of the rat's living during 5 weeks without decrease in the weight. Moreover, in the in-vivo experiments on the injection of the ESA-vesicle into the nude mouse carrying colo201, the growth inhibition of the colo201 was aiso observed. Thus, the ESA-vesicle is expected to using for the cancer therapy in DDS.
Next, some kinds of antibodies as a "missile device" as mentioned above were also immobilized on the Span80 vesicle. The specifical combines of the immunovesicles with each targeting cell were successfully confirmed the tageting experiments in vitro. The transfection of the DNA or prasmid entrapped in the immunovesicles to the targeting cells were also confirmed.
Thus, the above ESA-vesicles as well as an immunovesicle were expected to be applied to either DDS or gene transfection. Less
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